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It is very necessary to monitor H<jats:sub>2<\/jats:sub>S and relevant physiological processes in\u2005vivo. Herein, a new type of fluorophore with a reliable leaving group allows for excited\u2010state intramolecular transfer characteristics (ESIPT), inspired by mycophenolic acid. A morpholine ring was connected at the maleimide position of the probe to target the lysosome. Subsequently, the dinitrophenyl group known for a photoinduced electron transfer (PET) effect, was connected to allow for an effective \u201cturn\u2010on\u201d probe <jats:bold>Lyso\u2010H<jats:sub>2<\/jats:sub>S<\/jats:bold>. <jats:bold>Lyso\u2010H<jats:sub>2<\/jats:sub>S<\/jats:bold> demonstrated strong selectivity towards H<jats:sub>2<\/jats:sub>S, a large Stokes shift (111\u2005nm), and an incredibly low detection limit (41.8\u2005nM). The imaging of endogenous and exogenous H<jats:sub>2<\/jats:sub>S in living cells (A549\u2005cell line) was successfully achieved because of the specificity and ultra\u2010low toxicity (100\u2009% cell viability at 50\u2005\u03bcM concentration of <jats:bold>Lyso\u2010H<jats:sub>2<\/jats:sub>S<\/jats:bold>.) Additionally, <jats:bold>Lyso\u2010H<jats:sub>2<\/jats:sub>S<\/jats:bold> was also employed to visualize the activity of H<jats:sub>2<\/jats:sub>S in the gallbladder and intestine in a living zebrafish model. This is the first report of a fluorescent probe to track H<jats:sub>2<\/jats:sub>S sensing in specific organ systems to our knowledge.<\/jats:p>","DOI":"10.1002\/chem.202401733","type":"journal-article","created":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T10:09:38Z","timestamp":1719482978000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Lyso\u2010H<sub>2<\/sub>S: A Mycophenolic Acid\u2010Derived Probe for Ultra\u2010Low Toxicity, Intracellular H<sub>2<\/sub>S Detection, and Zebrafish Model Validation"],"prefix":"10.1002","volume":"30","author":[{"given":"Neha","family":"Jain","sequence":"first","affiliation":[{"name":"Department of Chemistry Korea Advanced Institute of Science and Technology (KAIST)  Daejeon 34141 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jongkeol","family":"An","sequence":"additional","affiliation":[{"name":"Department of Chemistry Korea Advanced Institute of Science and Technology (KAIST)  Daejeon 34141 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arkaprava","family":"Roychaudhury","sequence":"additional","affiliation":[{"name":"Department of Biology Chungnam National University  Daejeon 34134 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sujeong","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Chemistry Hallym University  Chuncheon 24252 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prasad M.","family":"Sonawane","sequence":"additional","affiliation":[{"name":"Department of Chemistry Korea Advanced Institute of Science and Technology (KAIST)  Daejeon 34141 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pheeranat","family":"Punyamung","sequence":"additional","affiliation":[{"name":"Department of Chemistry Korea Advanced Institute of Science and Technology (KAIST)  Daejeon 34141 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Aartsen","sequence":"additional","affiliation":[{"name":"Department of Chemistry Korea Advanced Institute of Science and Technology (KAIST)  Daejeon 34141 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satish Balasaheb","family":"Nimse","sequence":"additional","affiliation":[{"name":"Department of Chemistry Hallym University  Chuncheon 24252 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheol\u2010Hee","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biology Chungnam National University  Daejeon 34134 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2520-4638","authenticated-orcid":false,"given":"David G.","family":"Churchill","sequence":"additional","affiliation":[{"name":"Department of Chemistry Korea Advanced Institute of Science and Technology (KAIST)  Daejeon 34141 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,12]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1162667"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5309"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/ol400973v"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1506"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/c4cs00298a"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.90566.2008"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10895-022-02974-7"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2013.02.028"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/c4cs00298a"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201207701"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201104305"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6b04087"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/ol400973v"},{"key":"e_1_2_8_14_1","unstructured":"\u00a0"},{"key":"e_1_2_8_15_2","doi-asserted-by":"crossref","unstructured":"Pak Y.\u2005L. 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