{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T14:25:15Z","timestamp":1793024715384,"version":"4.5.0"},"reference-count":93,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T00:00:00Z","timestamp":1736726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","award":["CRG\/2022\/008554"],"award-info":[{"award-number":["CRG\/2022\/008554"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2025,2,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The recent surge in emerging viral infections warrants the design of broad\u2010spectrum antivirals. We aim to develop a lead molecule that targets a common biochemical feature of many enveloped viruses, membrane fusion. To achieve the broad\u2010spectrum ability, instead of targeting the fusion machinery, we plan to modulate the physicochemical properties of the host and viral membranes to block fusion. The approach is based on the Coronin\u20101 protein of <jats:italic>Mycobacterium<\/jats:italic>, which presumably inhibits the phagosome\u2010lysosome fusion, and a unique Trp\u2013Asp (WD) sequence is placed at the distorted \u03b2\u2010meander motif. We designed a WD\u2010based branched lipopeptide (Myr\u2010D(WD)<jats:sub>2<\/jats:sub>) that supported the intermolecular interactions to create a \u03b2\u2010sheet\u2010like supramolecular assembly at the membrane surface. TEM and confocal fluorescence experiments also suggest that the lipopeptide self\u2010assembled at the bilayer interface and modulated the interfacial order and the water penetration. We demonstrated that the supramolecular organization of Myr\u2010D(WD)<jats:sub>2<\/jats:sub> could block artificial membrane fusion completely and restrict pH\u2010dependent influenza virus (H1N1, H9N2), and pH\u2010independent mouse hepatitis virus, human coronavirus (HCoV\u2010OC43) infections. The present study provided an evidence\u2010based broad\u2010spectrum antiviral potential of a designed self\u2010assembled lipopeptide.<\/jats:p>","DOI":"10.1002\/chem.202403039","type":"journal-article","created":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T03:44:44Z","timestamp":1735011884000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>De Novo<\/i> Design of Lipopeptide\u2010Based \u03b2\u2010Sheet\u2010Like Self\u2010Assemblies: A Strategy to Develop Fusion Inhibitors as Broad\u2010Spectrum Antivirals"],"prefix":"10.1002","volume":"31","author":[{"given":"Avijit","family":"Sardar","sequence":"first","affiliation":[{"name":"Department of Chemical Sciences Indian Institute of Science Education and Research Kolkata  Mohanpur 741 246 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sucharita","family":"Bhowmick","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Indian Institute of Science Education and Research Kolkata  Mohanpur 741 246 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mithila","family":"Kamble","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Indian Institute of Science Education and Research Kolkata  Mohanpur 741 246 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikesh","family":"Dewangan","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences Indian Institute of Science Education and Research Kolkata  Mohanpur 741 246 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bibhas","family":"Hazra","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences Indian Institute of Science Education and Research Kolkata  Mohanpur 741 246 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2265-9856","authenticated-orcid":false,"given":"Amirul I.","family":"Mallick","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Indian Institute of Science Education and Research Kolkata  Mohanpur 741 246 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1059-950X","authenticated-orcid":false,"given":"Pradip K.","family":"Tarafdar","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences Indian Institute of Science Education and Research Kolkata  Mohanpur 741 246 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,13]]},"reference":[{"key":"e_1_2_12_1_1","unstructured":"\u00a0"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06536"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003467"},{"key":"e_1_2_12_4_1","unstructured":"World Health Organization Vol. 2024 2024."},{"key":"e_1_2_12_5_1","unstructured":"\u00a0"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abq3773"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-04389-z"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abn7842"},{"key":"e_1_2_12_9_1","unstructured":"Centers for Disease Control and Prevention Vol. 2024 2024."},{"key":"e_1_2_12_10_1","unstructured":"\u00a0"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/4581082a"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/486456a"},{"key":"e_1_2_12_13_1","unstructured":"\u00a0"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.13950"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)61684-5"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3475"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.02.054"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-380860-8.00004-5"},{"key":"e_1_2_12_19_1","unstructured":"\u00a0"},{"key":"e_1_2_12_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2021.183821"},{"key":"e_1_2_12_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2020.11.007"},{"key":"e_1_2_12_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aav4580"},{"key":"e_1_2_12_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abf4896"},{"key":"e_1_2_12_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00635-20"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.2c02501"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909587107"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41563-018-0194-2"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.0c06400"},{"key":"e_1_2_12_29_1","unstructured":"\u00a0"},{"key":"e_1_2_12_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1010026107"},{"key":"e_1_2_12_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02907-13"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202013848"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80754-0"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00232-022-00267-5"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja908560n"},{"key":"e_1_2_12_36_1","unstructured":"\u00a0"},{"key":"e_1_2_12_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23794-6"},{"key":"e_1_2_12_38_2","doi-asserted-by":"publisher","DOI":"10.3390\/app10175918"},{"key":"e_1_2_12_39_2","doi-asserted-by":"publisher","DOI":"10.1021\/bk-2000-0750.ch001"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/s22207874"},{"key":"e_1_2_12_41_1","unstructured":"\u00a0"},{"key":"e_1_2_12_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(01)76183-8"},{"key":"e_1_2_12_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2015.08.051"},{"key":"e_1_2_12_44_1","unstructured":"\u00a0"},{"key":"e_1_2_12_45_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.9b08455"},{"key":"e_1_2_12_46_2","doi-asserted-by":"publisher","DOI":"10.1021\/cn500231d"},{"key":"e_1_2_12_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2010.12.3692"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8CC00776D"},{"key":"e_1_2_12_49_1","unstructured":"\u00a0"},{"key":"e_1_2_12_50_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12551-020-00753-x"},{"key":"e_1_2_12_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2010.11.002"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2011.03.016"},{"key":"e_1_2_12_53_1","unstructured":"\u00a0"},{"key":"e_1_2_12_54_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201201661"},{"key":"e_1_2_12_55_2","doi-asserted-by":"publisher","DOI":"10.1039\/C8CC07207H"},{"key":"e_1_2_12_56_1","unstructured":"\u00a0"},{"key":"e_1_2_12_57_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja200310d"},{"key":"e_1_2_12_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2013.10.010"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2012.09.030"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2009.02.012"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.1021\/j100203a042"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2016.07.018"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2021.10.003"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.00116"},{"key":"e_1_2_12_65_1","unstructured":"\u00a0"},{"key":"e_1_2_12_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2017.03.010"},{"key":"e_1_2_12_67_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.77.070306.103621"},{"key":"e_1_2_12_68_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.0c07489"},{"key":"e_1_2_12_69_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.0c00502"},{"key":"e_1_2_12_70_1","unstructured":"\u00a0"},{"key":"e_1_2_12_71_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0701478104"},{"key":"e_1_2_12_72_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036833"},{"key":"e_1_2_12_73_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan0516"},{"key":"e_1_2_12_74_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar6221"},{"key":"e_1_2_12_75_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-020-0305-x"},{"key":"e_1_2_12_76_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00698-x"},{"key":"e_1_2_12_77_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00681-22"},{"key":"e_1_2_12_78_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1093646"},{"key":"e_1_2_12_79_1","unstructured":"\u00a0"},{"key":"e_1_2_12_80_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.1c00070"},{"key":"e_1_2_12_81_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.0c01136"},{"key":"e_1_2_12_82_1","unstructured":"\u00a0"},{"key":"e_1_2_12_83_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2022.198768"},{"key":"e_1_2_12_84_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108016"},{"key":"e_1_2_12_85_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1108777108"},{"key":"e_1_2_12_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.05.005"},{"key":"e_1_2_12_87_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.2c05759"},{"key":"e_1_2_12_88_1","unstructured":"\u00a0"},{"key":"e_1_2_12_89_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abd9909"},{"key":"e_1_2_12_90_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2300360120"},{"key":"e_1_2_12_91_1","first-page":"35","volume":"436","author":"Woolcock P. R.","year":"2008","journal-title":"Methods in Molecular Biology (Clifton, N.\u2005J.)"},{"key":"e_1_2_12_92_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"e_1_2_12_93_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04982-3"}],"container-title":["Chemistry \u2013 A European Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/chem.202403039","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T20:31:46Z","timestamp":1760301106000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/chem.202403039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,13]]},"references-count":93,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,2,20]]}},"alternative-id":["10.1002\/chem.202403039"],"URL":"https:\/\/doi.org\/10.1002\/chem.202403039","archive":["Portico"],"relation":{},"ISSN":["0947-6539","1521-3765"],"issn-type":[{"value":"0947-6539","type":"print"},{"value":"1521-3765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,13]]},"assertion":[{"value":"2024-10-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202403039"}}