{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T02:00:00Z","timestamp":1785463200844,"version":"3.56.0"},"reference-count":68,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T00:00:00Z","timestamp":1734652800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T00:00:00Z","timestamp":1734652800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["PID2021-123332OB-C21"],"award-info":[{"award-number":["PID2021-123332OB-C21"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["TED2021-129852B-C21;"],"award-info":[{"award-number":["TED2021-129852B-C21;"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["RT-134755-T"],"award-info":[{"award-number":["RT-134755-T"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014849","name":"Conselleria de Cultura, Educaci\u00f3n y Ciencia, Generalitat Valenciana","doi-asserted-by":"publisher","award":["CIPROM\/2021\/079"],"award-info":[{"award-number":["CIPROM\/2021\/079"]}],"id":[{"id":"10.13039\/501100014849","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004834","name":"Universitat Jaume I","doi-asserted-by":"publisher","award":["UJI-B-2022-12"],"award-info":[{"award-number":["UJI-B-2022-12"]}],"id":[{"id":"10.13039\/501100004834","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003086","name":"Eusko Jaurlaritza","doi-asserted-by":"publisher","award":["019-B1-2023"],"award-info":[{"award-number":["019-B1-2023"]}],"id":[{"id":"10.13039\/501100003086","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2025,1,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Regioselective oxidation of glyceryl alkyl ethers is of utmost importance for the fabrication of substituted hydroxy ketones and enantiopure 1,2\u2010diols as green solvents and pharmaceutical building blocks, respectively. An engineered glycerol dehydrogenase from\n                    <jats:italic>Bacillus stearothermophilus<\/jats:italic>\n                    was described to perform the regioselective oxidation of alkyl glycerol ethers, identifying position 252 as key for accepting larger substrates than glycerol. In this work, we further engineer that position through partial saturation mutagenesis to broaden the substrate scope toward other glycerol derivatives, improving enzyme kinetics and minimizing product inhibition. In particular, the BsGlyDH\u2212L252S variant becomes the most efficient biocatalyst for the deracemization of alkyl glyceryl ethers in a two\u2010step, one\u2010pot immobilized system. The discovery and use of these alternative mutants of GlyDH opens the road to more applications and increases the enzymatic toolbox for the modifications of glyceryl ethers.\n                  <\/jats:p>","DOI":"10.1002\/chem.202403735","type":"journal-article","created":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T07:23:02Z","timestamp":1733988182000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Active Site Engineering of a Glycerol Dehydrogenase Improves its Oxidative Activity and Scope Toward Glycerol Derivatives"],"prefix":"10.1002","volume":"31","author":[{"given":"Daniel A.","family":"Grajales\u2010Hern\u00e1ndez","sequence":"first","affiliation":[{"name":"Heterogeneous Biocatalysis Laboratory CICbiomaGUNE Basque Research and Technology Alliance (BRTA)  Paseo Miram\u00f3n, 194 Donostia-San Sebasti\u00e1n 20014 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maite","family":"Roca","sequence":"additional","affiliation":[{"name":"Institute of Advanced Materials (INAM) Universitat Jaume I  Av. Vicent Sos Baynat, s\/n Castell\u00f3 de la Plana 12071 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicent","family":"Moliner","sequence":"additional","affiliation":[{"name":"Institute of Advanced Materials (INAM) Universitat Jaume I  Av. Vicent Sos Baynat, s\/n Castell\u00f3 de la Plana 12071 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0031-1880","authenticated-orcid":false,"given":"Fernando","family":"L\u00f3pez\u2010Gallego","sequence":"additional","affiliation":[{"name":"Heterogeneous Biocatalysis Laboratory CICbiomaGUNE Basque Research and Technology Alliance (BRTA)  Paseo Miram\u00f3n, 194 Donostia-San Sebasti\u00e1n 20014 Spain"},{"name":"IKERBASQUE Basque Foundation for Science  Maria Diaz de Haro 3 Bilbao 48013 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,20]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetasy.2007.05.019"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph15020240"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1754-6834-5-48"},{"key":"e_1_2_8_4_1","unstructured":"\u00a0"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/md21010004"},{"key":"e_1_2_8_6_2","unstructured":"M. Miele L. Lelo V. Pace A.\u2005R. Alc\u00e1ntara inGreener Synthesis of Organic Compounds 1st Edition (Ed Ahindra Nag) CRC Press Boca Raton 2022 p. 29;"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2020.100281"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3GC41857J"},{"key":"e_1_2_8_9_1","unstructured":"\u00a0"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/cssc.202102628"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.7b00203"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-010-2735-8"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444994013533"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0SC04471G"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1507910112"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.3c02615"},{"key":"e_1_2_8_17_1","unstructured":"\u00a0"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.3c05749"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2010.09.027"},{"key":"e_1_2_8_20_1","unstructured":"\u00a0"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2009.07.001"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi991306p"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1980.tb04724.x"},{"key":"e_1_2_8_24_1","unstructured":"\u00a0"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2014.07.116"},{"key":"e_1_2_8_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.201500210"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/adsc.201801530"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcatb.2010.04.010"},{"key":"e_1_2_8_29_1","unstructured":"\u00a0"},{"key":"e_1_2_8_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2011.07.039"},{"key":"e_1_2_8_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.0c17568"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-11-101"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.3c15993"},{"key":"e_1_2_8_34_1","unstructured":"Schafmeister C. Ross W.S. Romanovski V. University of California San Francisco1995."},{"key":"e_1_2_8_35_1","unstructured":"I.\u2005Y.\u2005B.-S.\u2005D.\u2005A. Case S.\u2005R. Brozell D.\u2005S. Cerutti T.\u2005E. Cheatham V.\u2005W.\u2005D. Cruzeiro\u2005III T.\u2005A. Darden R.\u2005E. Duke D. Ghoreishi M.\u2005K. Gilson H. Gohlke A.\u2005W. Goetz D. Greene R. Harris N. Homeyer Y. Huang S. Izadi A. Kovalenko T. Kurtzman T.\u2005S. Lee S. LeGrand P. Li C. Lin J. Liu T. Luchko R. Luo D.\u2005J. Mermelstein K.\u2005M. Merz Y. Miao G. Monard C. Nguyen H. Nguyen I. Omelyan A. Onufriev F. Pan R. Qi D.\u2005R. Roe A. Roitberg C. Sagui S. Schott-Verdugo J. Shen C.\u2005L. Simmerling J. Smith R. Salomon-Ferrer J. Swails R.\u2005C. Walker J. Wang H. Wei R.\u2005M. Wolf X. Wu L. Xiao D.\u2005M. York P.\u2005A. Kollman AMBER 2018(Ed: U. o. California) University of California San Francisco2018."},{"key":"e_1_2_8_36_1","unstructured":"\u00a0"},{"key":"e_1_2_8_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.22102"},{"key":"e_1_2_8_38_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.448118"},{"key":"e_1_2_8_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20086"},{"key":"e_1_2_8_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetasy.2012.03.008"},{"key":"e_1_2_8_41_2","unstructured":"D.\u2005A. Case D.\u2005S. Cerutti T.\u2005E. Cheatham\u2005III T.\u2005A. Darden R.\u2005E. Duke T.\u2005J. Giese H. Gohlke A.\u2005W. Goetz N. Homeyer S. Izadi A. Kovalenko T.\u2005S. Lee S. LeGrand P. Li C. Lin J. Liu T. Luchko R. Luo D.\u2005J. Mermelstein K.\u2005M. Merz G. Monard H. Nguyen I. Omelyan A. Onufriev F. Pan R. Qi D.\u2005R. Roe A. Roitberg C. Sagui C.\u2005L. Simmerling W.\u2005M. Botello-Smith J. Swails R.\u2005C. Walker J. Wang R.\u2005M. Wolf X. Wu L. Xiao D.\u2005M. York P.\u2005A. Kollman University of California San Francisco2017;"},{"key":"e_1_2_8_42_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.464397"},{"key":"e_1_2_8_43_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.470117"},{"key":"e_1_2_8_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/1096-987X(200009)21:12<1088::AID-JCC5>3.0.CO;2-8"},{"key":"e_1_2_8_45_2","unstructured":"Gaussian 09 M. J. Frisch G.\u2005W. Trucks H.\u2005B. Schlegel G.\u2005E. Scuseria M.\u2005A. Robb J.\u2005R. Cheeseman G. Scalmani V. Barone B. Mennucci G.\u2005A. Petersson H. Nakatsuji M. Caricato X. Li H.\u2005P. Hratchian A.\u2005F. Izmaylov J. Bloino G. Zheng J.\u2005L. Sonnenberg M. Hada M. Ehara K. Toyota R. Fukuda J. Hasegawa M. Ishida T. Nakajima Y. Honda O. Kitao H. Nakai T. Vreven J.\u2005A. Montgomery\u2005Jr. J.\u2005E. Peralta F. Ogliaro M.\u2005J. Bearpark J.\u2005J. Heyd E.\u2005N. Brothers K.\u2005N. Kudin V.\u2005N. Staroverov R. Kobayashi J. Normand K. Raghavachari A.\u2005P. Rendell J.\u2005C. Burant S.\u2005S. Iyengar J. Tomasi M. Cossi N. Rega J.\u2005M. Millam M. Klene J.\u2005E. Knox J.\u2005B. Cross V. Bakken C. Adamo J. Jaramillo R. Gomperts R.\u2005E. Stratmann O. Yazyev A.\u2005J. Austin R. Cammi C. Pomelli J.\u2005W. Ochterski R.\u2005L. Martin K. Morokuma V.\u2005G. Zakrzewski G.\u2005A. Voth P. Salvador J.\u2005J. Dannenberg S. Dapprich A.\u2005D. Daniels \u00d6. Farkas J.\u2005B. Foresman J.\u2005V. Ortiz J. Cioslowski D.\u2005J. Fox Wallingford CT2009;"},{"key":"e_1_2_8_46_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevA.33.3628"},{"key":"e_1_2_8_47_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.445869"},{"key":"e_1_2_8_48_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.540130812"},{"key":"e_1_2_8_49_2","doi-asserted-by":"publisher","DOI":"10.1039\/C7GC01583F"},{"key":"e_1_2_8_50_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.20660"},{"key":"e_1_2_8_51_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.5b00674"},{"key":"e_1_2_8_52_2","doi-asserted-by":"publisher","DOI":"10.1021\/ct0501396"},{"key":"e_1_2_8_53_2","doi-asserted-by":"publisher","DOI":"10.1021\/ct100578z"},{"key":"e_1_2_8_54_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-987X(19970130)18:2<221::AID-JCC7>3.0.CO;2-X"},{"key":"e_1_2_8_55_2","doi-asserted-by":"publisher","DOI":"10.1021\/ct1002626"},{"key":"e_1_2_8_56_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp049633g"},{"key":"e_1_2_8_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(01)00645-1"},{"key":"e_1_2_8_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9991(77)90098-5"},{"key":"e_1_2_8_59_2","unstructured":"C.\u2005E.\u2005A.\u2005F. Schafmeister W. S. Ross V. Romanovski Universtity of California San Francisco.1995;"},{"key":"e_1_2_8_60_2","doi-asserted-by":"publisher","DOI":"10.1021\/ct200133y"},{"key":"e_1_2_8_61_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.540100208"},{"key":"e_1_2_8_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9991(77)90121-8"},{"key":"e_1_2_8_63_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp0261814"},{"key":"e_1_2_8_64_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi982057s"},{"key":"e_1_2_8_65_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20035"},{"key":"e_1_2_8_66_2","unstructured":"Schr\u00f6dinger L. & DeLano W.2020.PyMOL. Retrieved fromhttp:\/\/www.pymol.org\/pymol;"},{"key":"e_1_2_8_67_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.465608"},{"key":"e_1_2_8_68_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00214-007-0310-x"}],"container-title":["Chemistry \u2013 A European Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/chem.202403735","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/chem.202403735","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/chem.202403735","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T01:32:41Z","timestamp":1785461561000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/chem.202403735"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,20]]},"references-count":68,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,1,27]]}},"alternative-id":["10.1002\/chem.202403735"],"URL":"https:\/\/doi.org\/10.1002\/chem.202403735","archive":["Portico"],"relation":{},"ISSN":["0947-6539","1521-3765"],"issn-type":[{"value":"0947-6539","type":"print"},{"value":"1521-3765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,20]]},"assertion":[{"value":"2024-10-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202403735"}}