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Polyunsaturated fatty acids (PUFAs) are especially susceptible to such oxidative stress. This ROS\u2010induced lipid peroxidation is a key process of ferroptosis, an iron\u2010dependent, non\u2010apoptotic form of cell death. Although lipid peroxidation is an essential event in ferroptosis, current study tools for detecting the oxidation process lack specificity toward a particular membrane type such as plasma membrane. In this study, we present LipoxPM, a novel fluorescent probe that targets lipid peroxidation specifically in plasma membrane over other cellular membranes. LipoxPM features a BODIPY\u2010based fluorophore and an anionic sulfonate group, enhancing selective accumulation in the plasma membrane. LipoxPM was able to detect ferroptosis\u2010related lipid peroxidation in live cells, visualizing the spatial distribution of lipid peroxidation in the cell death processes. This probe would enable study of unknown mechanisms of ferroptosis and could potentially facilitate development of therapeutic strategies targeting oxidative stress\u2010related diseases.<\/jats:p>","DOI":"10.1002\/chem.202404323","type":"journal-article","created":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T22:16:52Z","timestamp":1739830612000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Visualization of Ferroptosis\u2010Induced Lipid Peroxidation Using Plasma Membrane\u2010Specific Fluorescent Probe LipoxPM"],"prefix":"10.1002","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6709-0897","authenticated-orcid":false,"given":"Hidefumi","family":"Iwashita","sequence":"first","affiliation":[{"name":"Department of Chemistry Faculty of Science Fukuoka University, Jonan-Ku  Fukuoka 814-0180 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