{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:26:59Z","timestamp":1772584019379,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T00:00:00Z","timestamp":1738281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22407130"],"award-info":[{"award-number":["22407130"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["224B101"],"award-info":[{"award-number":["224B101"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020197","name":"Postdoctoral Science Foundation of Shanghai Municipality","doi-asserted-by":"publisher","award":["2023692"],"award-info":[{"award-number":["2023692"]}],"id":[{"id":"10.13039\/501100020197","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2025,2,25]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The fluorescent imaging of pathologically accumulated \u03b2\u2010amyloid (A\u03b2) proteins is of significant importance to the diagnosis of Alzheimer's disease (AD). In the paper, we prepare two new NIR probes, <jats:bold>NIR\u20101<\/jats:bold> and <jats:bold>NIR\u20102<\/jats:bold>, through hydrophilic modification of introducing water\u2010soluble bioactive groups such as polyethylene glycol (PEG) and morpholine to tune <jats:italic>in\u2005vivo<\/jats:italic> pharmacokinetics for specific detection of soluble and insoluble A\u03b2 species. The <jats:italic>in\u2005vitro<\/jats:italic> assessments confirm that both <jats:bold>NIR\u20101<\/jats:bold> and <jats:bold>NIR\u20102<\/jats:bold> display strong near\u2010infrared (NIR) fluorescence (FL) enhancement upon interaction with A\u03b2<jats:sub>42<\/jats:sub> monomers, oligomers or aggregates (\u03bb<jats:sub>em<\/jats:sub>&gt;670\u2005nm) and show highly sensitive, rapid and selective response towards A\u03b2<jats:sub>42<\/jats:sub> species. After i.\u2009v. injection, each probe showed fast blood\u2010brain barrier (BBB) penetration and immediately produced intense FL signals in the brain of APP\/PS1 AD model mice at 10\u2005min. Moreover, compared with <jats:bold>NIR\u20102<\/jats:bold>, <jats:bold>NIR\u20101<\/jats:bold> bearing a hydrophilic PEG chain displayed not only more rapid clearance but also lower background signal to efficiently distinguish APP\/PS1 mice and wild\u2010type mice with higher signal\u2010to\u2010background ratio, which was further validated by <jats:italic>ex vivo<\/jats:italic> histological staining of brain tissues. Therefore, due to its excellent pharmacokinetics, <jats:bold>NIR\u20101<\/jats:bold> shows great promise as an effective NIR probe for specific detection of A\u03b2 species.<\/jats:p>","DOI":"10.1002\/chem.202404545","type":"journal-article","created":{"date-parts":[[2025,1,20]],"date-time":"2025-01-20T10:48:31Z","timestamp":1737370111000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development of Off\u2010On Near\u2010Infrared Fluorescent Probes for Sensitive <i>In Vivo<\/i> Imaging of Amyloid\u2010\u03b2 Species with Enhanced Pharmacokinetics"],"prefix":"10.1002","volume":"31","author":[{"given":"Qingchen","family":"Liu","sequence":"first","affiliation":[{"name":"School of Pharmacy China Pharmaceutical University  Nanjing 211198 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiming","family":"Men","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Analytical Chemistry for Life Science Chemistry and Biomedicine Innovation Center (ChemBIC) School of Chemistry and Chemical Engineering Nanjing University  Nanjing 210023 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daqing","family":"Fang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinxing","family":"Miao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Analytical Chemistry for Life Science Chemistry and Biomedicine Innovation Center (ChemBIC) School of Chemistry and Chemical Engineering Nanjing University  Nanjing 210023 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9887-0914","authenticated-orcid":false,"given":"Deju","family":"Ye","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Analytical Chemistry for Life Science Chemistry and Biomedicine Innovation Center (ChemBIC) School of Chemistry and Chemical Engineering Nanjing University  Nanjing 210023 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy China Pharmaceutical University  Nanjing 211198 China"},{"name":"State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,31]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)32205-4"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2006-9S317"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-021-01249-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.15007"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc-2016-0185"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2015.56"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2024.117546"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8CS00986D"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222312768"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.0c01101"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.3c00304"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2AN01327D"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.acc.2020.08.008"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dyepig.2024.111993"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.3c01057"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202216351"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202211550"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/cbmi.3c00081"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.3c09034"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11426-021-1113-0"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b12820"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27123879"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.10.047"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.0c01914"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.4c00252"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja405239v"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1505420112"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.1c00419"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.68679"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-018-0005-0"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2014.06.046"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.9b00707"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0TB02911D"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nucmedbio.2005.06.001"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.8b01712"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.0c00729"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1214134109"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr0782426"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.7b01834"},{"key":"e_1_2_7_40_1","unstructured":"M. Rana K. Terpstra C. Gutierrez K. Xu H. Arya T.\u2005K. Bhatt L.\u2005M. Mirica A.\u2005K. Sharma Chem. Eur. J.2024 e202402748."},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11307-023-01800-1"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm030026b"}],"container-title":["Chemistry \u2013 A European Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/chem.202404545","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T16:46:13Z","timestamp":1760287573000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/chem.202404545"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,31]]},"references-count":42,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,2,25]]}},"alternative-id":["10.1002\/chem.202404545"],"URL":"https:\/\/doi.org\/10.1002\/chem.202404545","archive":["Portico"],"relation":{},"ISSN":["0947-6539","1521-3765"],"issn-type":[{"value":"0947-6539","type":"print"},{"value":"1521-3765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,31]]},"assertion":[{"value":"2024-12-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202404545"}}