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Biosensors utilizing 2D nanomaterials enable real\u2010time disease monitoring, allowing for prompt intervention and therapy, tracking of physiological activities and enhancing the understanding of the human biosystem. Since they are cost\u2010effective and user\u2010friendly, 2D nanomaterials have been utilized in commercial biosensors. Over the past decade, graphene and its derivatives have been the most studied 2D nanomaterials, due to their large surface area and tuneable electronic and optical properties, which significantly enhance their sensitivity and specificity. Nevertheless, recently discovered 2D nanomaterials, including MXenes, black phosphorus and hexagonal boron nitride, remain largely unexplored. Despite their numerous applications, the broader potential of these materials in biosensing remains insufficiently explored in current literature. This review offers a comprehensive perspective by specifically focusing on inorganic 2D nanomaterials, an area that remains underrepresented in the literature. 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