{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:42:04Z","timestamp":1760316124749,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"47","license":[{"start":{"date-parts":[[2025,7,26]],"date-time":"2025-07-26T00:00:00Z","timestamp":1753488000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,7,26]],"date-time":"2025-07-26T00:00:00Z","timestamp":1753488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJPR2143"],"award-info":[{"award-number":["JPMJPR2143"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2025,8,21]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The NELL2\u2010interacting cofactor for lumicrine signaling (NICOL) is a small protein which forms a complex with the neural epidermal growth factor\u2013like like 2 (NELL2) and engaged in sperm maturation in the epididymis. At present, the recombinant expression of NICOL is not efficient, which limits the precise understanding of the structure and function of NICOL. In this study, we first succeeded in the chemical synthesis of NICOL with NELL2 binding affinity. The synthesized NICOL possesses correct SS bond pairing, and its secondary structure is indistinguishable from that of the recombinant protein, indicating that the synthetic NICOL will be useful for structural and functional analyses of lumicrine signaling.<\/jats:p>","DOI":"10.1002\/chem.202501588","type":"journal-article","created":{"date-parts":[[2025,7,26]],"date-time":"2025-07-26T13:06:21Z","timestamp":1753535181000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemical Synthesis of NICOL, A Coligand Secreted Protein Mediating Mammalian Male Reproductive Tract Trans\u2010luminal Signaling"],"prefix":"10.1002","volume":"31","author":[{"given":"Kenta","family":"Nagahama","sequence":"first","affiliation":[{"name":"Institute for Protein Research The University of Osaka  3\u20102 Yamadaoka Suita Osaka 565\u20130871 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiki","family":"Takei","sequence":"additional","affiliation":[{"name":"Institute for Protein Research The University of Osaka  3\u20102 Yamadaoka Suita Osaka 565\u20130871 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shun","family":"Ito","sequence":"additional","affiliation":[{"name":"Institute for Protein Research The University of Osaka  3\u20102 Yamadaoka Suita Osaka 565\u20130871 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshifumi","family":"Takao","sequence":"additional","affiliation":[{"name":"Institute for Protein Research The University of Osaka  3\u20102 Yamadaoka Suita Osaka 565\u20130871 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0916-1233","authenticated-orcid":false,"given":"Hironobu","family":"Hojo","sequence":"additional","affiliation":[{"name":"Institute for Protein Research The University of Osaka  3\u20102 Yamadaoka Suita Osaka 565\u20130871 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daiji","family":"Kiyozumi","sequence":"additional","affiliation":[{"name":"Research Organization of Science and Technology Ritsumeikan University  1\u20101\u20101, Noji\u2010higashi Kusatsu Shiga 525\u20138577 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,26]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12632"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/gtc.13066"},{"key":"e_1_2_9_3_1","first-page":"1","volume":"21","author":"James E. 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