{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T02:31:37Z","timestamp":1791685897855,"version":"4.3.4"},"reference-count":26,"publisher":"Wiley","issue":"63","license":[{"start":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T00:00:00Z","timestamp":1757289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2025,11,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The long\u2010term visualization of intracellular Fe\n                    <jats:sup>3+<\/jats:sup>\n                    dynamics and lysosomal activity is crucial for investigating the physiological roles and functions of lysosomes during the growth of organisms. The lysosome\u2010targeted fluorescent probe (RBH\u2010EdC), derived from rhodamine\u2010nucleoside conjugates, demonstrates a sophisticated dual\u2010activation design: one is Fe\n                    <jats:sup>3<\/jats:sup>\n                    \u207a response, triggering spirolactam ring\u2010opening to form xanthine structures, resulting in\u00a0\u2265\u00a01000\u2010fold fluorescence enhancement with visible colorimetric transition (colorless\u2192pink). Another is pH sensitivity, demonstrating protonation\u2010dependent fluorescence amplification at the dC at site N3 (pK\n                    <jats:sub>a\u00a0<\/jats:sub>\n                    =\u00a02.9), achieving a 30\u2010fold intensity increase at pH 2.5 within the 1.0\u20135.0 range. Remarkably, the RBH\u2010EdC\/Fe\n                    <jats:sup>3<\/jats:sup>\n                    \u207a complex showed a further significant 20\u2010fold fluorescence enhancement at pH 3.0. This Fe\n                    <jats:sup>3<\/jats:sup>\n                    \u207a\/H\u207a co\u2010activated \u201cturn\u2010on\u201d system provides exceptional signal amplification for both in vitro and in vivo applications, enabling precise exploration of lysosome\u2010related biological processes, including the response of the pH microenvironment, real\u2010time monitoring of lysosomal pH dynamics, in situ measurement of gastric acidity levels, and potential application in gastric ulcer diagnostics.\n                  <\/jats:p>","DOI":"10.1002\/chem.202501717","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T05:47:57Z","timestamp":1757396877000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Fe\n                    <sup>3+<\/sup>\n                    and H\n                    <sup>+<\/sup>\n                    \u2010triggered \u201cTurn\u2010on\u201d Fluorescence Probe Based on Lysosomal\u2010targeted RBH\u2010EdC for Monitoring Intragastric Acidity"],"prefix":"10.1002","volume":"31","author":[{"given":"Weiqing","family":"Xu","sequence":"first","affiliation":[{"name":"Institute for Advanced Study Shenzhen University  Shenzhen 518060 P. 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