{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T22:04:27Z","timestamp":1778709867044,"version":"3.51.4"},"reference-count":85,"publisher":"Wiley","issue":"63","license":[{"start":{"date-parts":[[2025,9,6]],"date-time":"2025-09-06T00:00:00Z","timestamp":1757116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2025,11,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Nitric oxide (NO) is one of the crucial biological signaling molecules, yet achieving its selective and spatiotemporal detection in in\u2010situ\/invitro or biological systems at specific pH remains a significant challenge. Hence, a probe capable of directly detecting NO would be immensely valuable in understanding its reactivity and biological functions. Here, to develop a Cu(II)\u2010based probe for selective NO detection, we synthesized a Cu(II)\u2010complex (\n                    <jats:bold>1<\/jats:bold>\n                    ) using a N3\u2010tridentate ligand having a pendant dansyl fluorophore (L\n                    <jats:sub>1<\/jats:sub>\n                    ) and evaluated it's NO reactivity under varying pH conditions. The binding of Cu(II) ion with ligand L\n                    <jats:sub>1<\/jats:sub>\n                    to prepare\n                    <jats:bold>1<\/jats:bold>\n                    showed\u00a0&gt;\u00a096% fluorescence quenching (fluorescence turn\u2010off) compared to highly fluorescent free L\n                    <jats:sub>1<\/jats:sub>\n                    (\u03a6 = 59%). Complex\n                    <jats:bold>1<\/jats:bold>\n                    did not react with NO in neutral or acidic medium; however, in the presence of onefold base (pH\u00a0\u2265\u00a09), it showed the reductive nitrosylation reaction, which resulted in the reduction of Cu(II) to Cu(I) and ligand nitrosylation (L\n                    <jats:sub>1<\/jats:sub>\n                    \u2010NO) with fluorescence turn\u2010on. Studies performed using different reactive nitrogen \/or oxygen species (RNS\/ROS) suggest that probe 1 is highly selective toward NO with a detection limit of\n                    <jats:bold>1<\/jats:bold>\n                    \u00a0nM. For the first time, the base\u2010catalyzed reductive nitrosylation of a Cu(II) complex resulted in the selective NO detection via fluorescence turn\u2010on at pH\u00a0\u2265\u00a09 in methanol \/or aqueous buffer medium. To understand the effect of secondary amine (\u2500NH\u2500) sites in the above reductive nitrosylation reaction, a Cu(II) complex (\n                    <jats:bold>2<\/jats:bold>\n                    ) of the ligand (L\n                    <jats:sub>2<\/jats:sub>\n                    ), having two different (\u2500NH\u00a0sites, was reacted with NO under various pH conditions. Surprisingly,\n                    <jats:bold>2<\/jats:bold>\n                    showed selective mono\u2010N\u2010nitrosylation of the amine site coordinated to Cu(II) ion with the reduction of Cu(II) in the presence of onefold OH\n                    <jats:sup>\u2212<\/jats:sup>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/chem.202501833","type":"journal-article","created":{"date-parts":[[2025,9,6]],"date-time":"2025-09-06T07:48:52Z","timestamp":1757144932000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["pH\u2010Responsive Fluorescence Turn\u2010On Copper(II)\u2010Probe for Nitric Oxide Detection: A Reductive Nitrosylation Approach"],"prefix":"10.1002","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-2863-6411","authenticated-orcid":false,"given":"Sanjai","family":"Muthusamy","sequence":"first","affiliation":[{"name":"IISER Tirupati: Indian Institute of Science Education and Research Tirupati Tirupati 517619 INDIA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0815-4170","authenticated-orcid":false,"given":"Soumak","family":"Mahato","sequence":"additional","affiliation":[{"name":"IISER Tirupati: Indian Institute of Science Education and Research Tirupati Tirupati 517619 INDIA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9987-8635","authenticated-orcid":false,"given":"Prabhakar","family":"Bhardwaj","sequence":"additional","affiliation":[{"name":"IISER Tirupati: Indian Institute of Science Education and Research Tirupati Tirupati 517619 INDIA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0164-5675","authenticated-orcid":false,"given":"Ruppa Durga","family":"Rao","sequence":"additional","affiliation":[{"name":"IISER Tirupati: Indian Institute of Science Education and Research Tirupati Tirupati 517619 INDIA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6395-9257","authenticated-orcid":false,"given":"Kalai Selvan","family":"Karumban","sequence":"additional","affiliation":[{"name":"IISER Tirupati: Indian Institute of Science Education and Research Tirupati Tirupati 517619 INDIA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2530-7386","authenticated-orcid":false,"given":"Pankaj","family":"Kumar","sequence":"additional","affiliation":[{"name":"IISER Tirupati: Indian Institute of Science Education and Research Tirupati Tirupati 517619 INDIA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,6]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020150124721"},{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-3773(19990712)38:13\/14<1870::AID-ANIE1870>3.0.CO;2-8"},{"key":"e_1_2_8_1_3","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-3773(19990712)38:13\/14<1856::AID-ANIE1856>3.0.CO;2-D"},{"key":"e_1_2_8_1_4","doi-asserted-by":"publisher","DOI":"10.1038\/327524a0"},{"key":"e_1_2_8_1_5","doi-asserted-by":"publisher","DOI":"10.1039\/cs9932200233"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/347768a0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(00)00044-7"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007604414773"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/19.5.711"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005960822365"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2222"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio1105-308"},{"key":"e_1_2_8_6_3","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200400271"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2005.05.007"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2013.01.006"},{"key":"e_1_2_8_7_3","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-010-0808-2"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-anchem-060908-155146"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr010152s"},{"key":"e_1_2_8_8_3","first-page":"31","volume":"1998","author":"Mao L.","year":"1991","journal-title":"Anal. 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