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In this study, we developed tCAP(N3), a novel traceless chemical conjugation method that employs an affinity peptide. tCAP(N3) can be stored for over a year at refrigerated temperature, as it contains an active ester precursor that can be spontaneously activated under neutral conditions. When tCAP(N3) was mixed with a target native IgG, Ac\u2010Lys(N\n                    <jats:sub>3<\/jats:sub>\n                    )\u2010Gly\u2010Gly was site\u2010selectively transferred onto Lys248, yielding divalently azidated IgG that can be further modified with DBCO compounds. The resulting antibody conjugates retained Fc receptor binding and antigen recognition comparable to the parent IgG.\n                  <\/jats:p>","DOI":"10.1002\/chem.202503144","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T15:13:24Z","timestamp":1766589204000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development of tCAP(N3): Affinity Peptide\u2010Aided, pH\u2010Triggered Strategy for Site\u2010Specific Native IgG Modification"],"prefix":"10.1002","volume":"32","author":[{"given":"Hiroko","family":"Kawakami","sequence":"first","affiliation":[{"name":"Graduate School of Science and Engineering Kagoshima University  Kagoshima Japan"},{"name":"Peptide Institute, Inc.  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