{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T16:41:07Z","timestamp":1781541667790,"version":"3.54.5"},"reference-count":56,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2026,5,22]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A shape\u2010transforming three\u2010dimensional DNA nanoscaffold was constructed to mimic the confined microenvironment of a carboxysome for carbon dioxide (CO\n                    <jats:sub>2<\/jats:sub>\n                    ) fixation. The DNA scaffold, designed as a shallow hexagonal prism (SHP), exhibited a structural transition between open and closed states through DNA linker hybridization, allowing direct comparison of identical enzyme populations under distinct microenvironments. Ribulose\u20101,5\u2010bisphosphate carboxylase\/oxygenase (RuBisCO) was covalently immobilized onto the SHP through a modular CLIP\u2010GCN4 adaptor composed of a CLIP\u2010tag and GCN4 peptide. The quantitative and site\u2010specific assembly of the adaptor\u2010fused RuBisCO (CG\u2010RuBisCO) was confirmed by AFM imaging and electrophoretic mobility assays. The efficient structural transformation from the open to closed state was verified by fluorescence resonance energy transfer analysis. In the closed state, RuBisCO enzymes were brought into self\u2010contacting proximity, thereby mimicking the natural carboxysomal environment at the molecular level. A quantitative comparison between the CO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010fixing reaction in the open and closed states revealed that the profiles for both states were comparable. This reconfigurable DNA\u2010based compartment enables quantitative control over the number of enzymes and their spatial proximity, providing a versatile platform that facilitates the analysis of the effects of nanoscale confinement and spatial organization of enzymes on metabolic efficiency within synthetic microcompartments.\n                  <\/jats:p>","DOI":"10.1002\/chem.202503305","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T11:02:33Z","timestamp":1772967753000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Construction of a CO\n                    <sub>2<\/sub>\n                    \u2010Fixing Compartment Using a Shape\u2010Transforming DNA Scaffold"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8521-4043","authenticated-orcid":false,"given":"Hui","family":"Yang","sequence":"first","affiliation":[{"name":"Institute of Advanced Energy Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2601-0787","authenticated-orcid":false,"given":"Peng","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Advanced Energy Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3368-9951","authenticated-orcid":false,"given":"Surachada","family":"Chuaychob","sequence":"additional","affiliation":[{"name":"Institute of Advanced Energy Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0336-0362","authenticated-orcid":false,"given":"Huyen","family":"Dinh","sequence":"additional","affiliation":[{"name":"Institute of Advanced Energy Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Nakabayashi","sequence":"additional","affiliation":[{"name":"Institute of Advanced Energy Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9564-6805","authenticated-orcid":false,"given":"Eiji","family":"Nakata","sequence":"additional","affiliation":[{"name":"Institute of Advanced Energy Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3663-3267","authenticated-orcid":false,"given":"Takashi","family":"Morii","sequence":"additional","affiliation":[{"name":"Institute of Advanced Energy Kyoto University  Kyoto Japan"},{"name":"Department of Health and Nutrition Kyoto Koka Women's University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1017150108"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1172046"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.48480"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.06.010"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1374-1"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature22822"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.02.007"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.08.048"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.11.054"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2014.10.076"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.7"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2016.05.042"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.03.046"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2308600120"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1108557109"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M709285200"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.8b06822"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2014406118"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0880-5"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.03629-21"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2019.115120"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.6b04744"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1CC00876E"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.139"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4071"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202002740"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.0c00235"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10619"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.9b05746"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4CC08472A"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01072-8"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1557\/mrs.2017.269"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja300897h"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep39587"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.9b01857"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1NR02873A"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.1c01763"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08016"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1214081"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2021.697857"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202215023"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1CC00276G"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1CC04162B"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2024.05.007"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-32584-7"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4CC08167F"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2013.10.014"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.7b01640"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9SC02990G"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14472"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0SC03498C"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/cs300766d"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1139\/w04-106"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16580-3"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202103304"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(74)90274-5"}],"container-title":["Chemistry \u2013 A European Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/chem.202503305","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/chem.202503305","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/chem.202503305","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T10:37:08Z","timestamp":1781519828000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/chem.202503305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,2]]},"references-count":56,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2026,5,22]]}},"alternative-id":["10.1002\/chem.202503305"],"URL":"https:\/\/doi.org\/10.1002\/chem.202503305","archive":["Portico"],"relation":{},"ISSN":["0947-6539","1521-3765"],"issn-type":[{"value":"0947-6539","type":"print"},{"value":"1521-3765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,2]]},"assertion":[{"value":"2025-10-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03305"}}