{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T17:53:40Z","timestamp":1768067620284,"version":"3.49.0"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2026,1,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The enantioselective conjugate hydrocyanation of\n                    <jats:italic>\u03b1<\/jats:italic>\n                    ,\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010unsaturated aldehydes remains a long\u2010standing challenge in synthetic chemistry. Here, we report the redesign of 2\u2010deoxy\u2010D\u2010ribose\u20105\u2010phosphate aldolase (DERA) into an efficient biocatalyst capable of promoting the asymmetric conjugate addition of hydrogen cyanide (generated in situ from trimethylsilyl cyanide) to aromatic enals via an iminium activation pathway. The evolved variant DERA\u2010CN enables the efficient formation of various C4\u2010nitriles with high conversions (up to 99%) and good enantioselectivity (up to 98% e.e.). Control experiments revealed a stepwise process involving enzyme\u2010catalyzed conjugate hydrocyanation followed by spontaneous 1,2\u2010addition of cyanide. Substrates with various electron\u2010donating and electron\u2010withdrawing groups are tolerated, providing access to various enantioenriched nitriles. This work expands the scope of DERA\u2010promoted iminium catalysis and provides a rare enzymatic platform for asymmetric conjugate hydrocyanation under mild aqueous conditions.\n                  <\/jats:p>","DOI":"10.1002\/chem.202503435","type":"journal-article","created":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T03:56:43Z","timestamp":1765598203000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Asymmetric Conjugate Hydrocyanation of\n                    <i>\u03b1<\/i>\n                    ,\n                    <i>\u03b2<\/i>\n                    \u2010Unsaturated Aldehydes Catalyzed by Engineered 2\u2010Deoxy\u2010D\u2010ribose\u20105\u2010phosphate Aldolase"],"prefix":"10.1002","volume":"32","author":[{"given":"Hangyu","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy University of Groningen  Groningen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Fodran","sequence":"additional","affiliation":[{"name":"Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy University of Groningen  Groningen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haigen","family":"Fu","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Biotechnology for Microbial Drugs, Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerrit J.","family":"Poelarends","sequence":"additional","affiliation":[{"name":"Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy University of Groningen  Groningen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432\u20101033.1982.tb06719.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1063601"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr0684016"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.1c03911"},{"key":"e_1_2_8_6_1","first-page":"79","volume":"39","author":"Lelais G.","year":"2006","journal-title":"Aldrichimica 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