{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T01:13:07Z","timestamp":1790039587351,"version":"4.0.1"},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T00:00:00Z","timestamp":1768435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T00:00:00Z","timestamp":1768435200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["206021_213224"],"award-info":[{"award-number":["206021_213224"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2026,3,16]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Natural isonitriles are promising lead compounds for medicinal chemistry. Their identification is, however, challenging due to the hydrolytic lability of the isonitrile group. Here, we use the azomethine imine (AMI)\u2013isonitrile ligation in a reactivity\u2010based screening protocol for the chemoselective derivatization of natural isonitriles. The herein developed AMI probe rapidly reacts with isonitriles\u2014primary, secondary, tertiary, as well as aromatic\u2014to stable conjugates. A dibromide mass tag enables the detection of low\u2010abundance isonitriles, even in complex biological matrices. The ligation establishes a new stereogenic center, thereby allowing facile distinction between achiral and chiral isonitriles by the formation of racemates or diastereoisomers, respectively. In addition, a unique reactivity of isonitriles bearing an \u03b1\u2010COOH group was unraveled. This AMI probe enabled the detection of known bacterially produced isonitriles and the identification of a novel fungal isonitrile.<\/jats:p>","DOI":"10.1002\/chem.202503642","type":"journal-article","created":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T06:01:48Z","timestamp":1768543308000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Identification of Natural Isonitriles Through Ligation to an Azomethine Imine Probe"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2687-9055","authenticated-orcid":false,"given":"Maurice P.","family":"Biedermann","sequence":"first","affiliation":[{"name":"Laboratory of Organic Chemistry D\u2010CHAB ETH Z\u00fcrich  Z\u00fcrich 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