{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T13:05:25Z","timestamp":1789736725542,"version":"4.0.1"},"reference-count":42,"publisher":"Wiley","issue":"35","license":[{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201020\u2010CE13\u20100017"],"award-info":[{"award-number":["ANR\u201020\u2010CE13\u20100017"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2026,9,19]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>We present a modular biomimetic fluorescent sensing platform designed to precisely interrogate the physicochemical mechanisms governing phagosomal acidification. Phagocytosis is an internalization process for micrometric biological objects and the later stages of phagosomal maturation \u2010including acidification\u2010 play a pivotal role in pathogen destruction and immune signaling. In vitro studies with particulate pathogen models have shown that these degradation pathways depend on the nature of the internalized object as well as on the type of receptors engaged. To alleviate this variability and gain a finer understanding of the phagosome maturation, microparticulate fluorescent pH sensors with chemically controlled composition and cell\u2010like mechanical properties are thus still lacking. The soft particle\u2010based biosensors presented herein aim at filling this gap by uniquely integrating (i) cell\u2010mimicking properties with a fluid and deformable interface, (ii) receptor\u2010specific uptake and (iii) a ratiometric pH\u2010responsive fluorescence to quantitatively follow the onset and progression of phagosomal acidification. This versatile platform provides a direct means to couple defined interfacial recognition events to intracellular pH measurements, offering new opportunities to unravel phagosomal physiology.<\/jats:p>","DOI":"10.1002\/chem.71237","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T13:01:57Z","timestamp":1780923717000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ratiometric pH\u2010Responsive and Biomimetic Microparticles for Quantitative Monitoring of Phagosomal Acidification"],"prefix":"10.1002","volume":"32","author":[{"given":"Sophie","family":"Michelis","sequence":"first","affiliation":[{"name":"Laboratoire CPCV UMR8228, D\u00e9partement de Chimie, \u00c9cole normale sup\u00e9rieure, PSL University, Sorbonne Universit\u00e9 CNRS  Paris 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