{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T13:46:09Z","timestamp":1791899169332,"version":"4.3.4"},"reference-count":60,"publisher":"Wiley","issue":"38","license":[{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006445","name":"Uniwersytet Warszawski","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006445","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2026,10,13]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Adenosine triphosphate (ATP), a common constituent of protein\u2010rich biomolecular condensates, itself undergoes liquid\u2013liquid phase separation (LLPS) even in the absence of polypeptides. Using polyethylene glycol as macromolecular crowding agent, we observed robust ATP droplet formation at pH 2\u201311. Moderate NaCl concentrations and lower temperatures promote LLPS by lowering critical ATP and crowder concentrations. While very high salt reverses this trend, the droplets nevertheless survive in hypersaline environments. We argue that both ion\u2010mediated bridging and anomalous underscreening likely contribute to the response of ATP droplets to increasing ionic strength. Most importantly, physiological millimolar ATP concentrations are sufficient for phase separation in the presence of millimolar Mg\n                    <jats:sup>2<\/jats:sup>\n                    <jats:sup>+<\/jats:sup>\n                    and crowders, mimicking intracellular conditions. pH tunes intermolecular interactions, evidenced by inversion of the adenosine circular dichroism (CD) signal. These results reveal intrinsic, protein\u2010independent LLPS of ATP with potential roles in cellular compartmentalization and pathological phase transitions.\n                  <\/jats:p>","DOI":"10.1002\/chem.71374","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T17:44:41Z","timestamp":1783100681000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protein\u2010Independent Liquid\u2013Liquid Phase Separation of Adenosine Triphosphate Under Crowded Conditions"],"prefix":"10.1002","volume":"32","author":[{"given":"Robert","family":"Dec","sequence":"first","affiliation":[{"name":"Physical Chemistry I \u2013 Biophysical Chemistry, Department of Chemistry and Chemical Biology TU Dortmund University  Dortmund Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wojciech","family":"Dzwolak","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Biological and Chemical Research Centre University of Warsaw  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Winter","sequence":"additional","affiliation":[{"name":"Physical Chemistry I \u2013 Biophysical Chemistry, Department of Chemistry and Chemical Biology TU Dortmund University  Dortmund Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00814"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav6691"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-0272-6"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.09.015"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.18413"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1172046"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100913-013325"},{"key":"e_1_2_8_9_1","volume-title":"The Origin of Life on the Earth","author":"Oparin A. 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