{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T10:50:37Z","timestamp":1785754237531,"version":"3.56.0"},"reference-count":56,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["565232660"],"award-info":[{"award-number":["565232660"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry A European J"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Metabolic enzymes can operate in concerted networks, so\u2010called metabolons. The glucosome is a multi\u2010enzyme complex that includes enzymes of glycolysis and gluconeogenesis and regulates glucose metabolism in mammalian cells. The mechanism of how glucosomes assemble remains largely unknown. Fluorescence recovery after photobleaching (FRAP) experiments suggest that glucosomes are liquid\u2010like, but direct evidence of a phase transition mechanism is missing. Here, we developed a novel method to measure the phase transition of condensates in living cells by combining infrared laser heating with confocal microscopy of fluorescently labeled phosphofructokinase 1 liver\u2010type (PFKL). We showed that glucosomes form and dissolve reversibly in HeLa cells upon temperature modulation, allowing us to determine the condensation temperature. We studied cells under osmotic stress to show that glucosomes retain their phase behavior despite a significant decrease in the mobility of PFKL. We propose that this method is a versatile tool to study the phase behavior of biomolecular condensates, yielding complementary information to well\u2010established techniques such as FRAP.<\/jats:p>","DOI":"10.1002\/chem.71500","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T10:42:31Z","timestamp":1785753751000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Infrared Laser Heating Reveals a Reversible Phase Transition of Phosphofructokinase 1 in Mammalian Cells"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9678-7870","authenticated-orcid":false,"given":"Morten","family":"Mosbach","sequence":"first","affiliation":[{"name":"Lehrstuhl f\u00fcr Biophysikalische Chemie and Research Center Chemical Sciences and Sustainability Research Alliance Ruhr  Bochum Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5626-6173","authenticated-orcid":false,"given":"Sneha Pavanan","family":"Kuruvanthara","sequence":"additional","affiliation":[{"name":"Lehrstuhl f\u00fcr Biophysikalische Chemie and Research Center Chemical Sciences and Sustainability Research Alliance Ruhr  Bochum Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2392-6897","authenticated-orcid":false,"given":"Lukas","family":"Johannknecht","sequence":"additional","affiliation":[{"name":"Lehrstuhl f\u00fcr Biophysikalische Chemie and Research Center Chemical Sciences and Sustainability Research Alliance Ruhr  Bochum Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9309-1279","authenticated-orcid":false,"given":"Simon","family":"Ebbinghaus","sequence":"additional","affiliation":[{"name":"Lehrstuhl f\u00fcr Biophysikalische Chemie and Research Center Chemical Sciences and Sustainability Research Alliance Ruhr  Bochum Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"2553","DOI":"10.1021\/acs.chemrev.3c00681","article-title":"Exploring Emergent Properties in Enzymatic Reaction Networks: Design and Control of Dynamic Functional Systems","volume":"124","author":"Ghosh S.","year":"2024","journal-title":"Chemical Reviews"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbior.2025.101105","article-title":"Unveiling the Role of Biomolecular Condensates in Cellular Function and Cancer","volume":"98","author":"Elena H.\u2010L.","year":"2025","journal-title":"Advances in Biological Regulation"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1038\/nrm.2017.7","article-title":"Biomolecular Condensates: Organizers of Cellular Biochemistry","volume":"18","author":"Banani S. F.","year":"2017","journal-title":"Nature Reviews Molecular Cell Biology"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"1786","DOI":"10.1016\/S0021-9258(19)75707-X","article-title":"Further Characterization of the Krebs Tricarboxylic Acid Cycle Metabolon","volume":"262","author":"Robinson J. B.","year":"1987","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1126\/science.1152241","article-title":"Reversible Compartmentalization of De Novo Purine Biosynthetic Complexes in Living Cells","volume":"320","author":"An S.","year":"2008","journal-title":"Science"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/0003-9861(90)90284-6","article-title":"Glucose Metabolism and the Channeling of Glycolytic Intermediates in Permeabilized L\u2010929 Cells","volume":"278","author":"Clegg J. S.","year":"1990","journal-title":"Archives of Biochemistry and Biophysics"},{"key":"e_1_2_8_8_1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/0968-0004(85)90266-X","article-title":"The Metabolon","volume":"10","author":"Srere P. A.","year":"1985","journal-title":"Trends in Biochemical Sciences"},{"key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1006\/meth.1999.0858","article-title":"Substrate Channeling","volume":"19","author":"Spivey H. O.","year":"1999","journal-title":"Methods"},{"key":"e_1_2_8_10_1","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1038\/nchem.2459","article-title":"Substrate Channelling as an Approach to Cascade Reactions","volume":"8","author":"Wheeldon I.","year":"2016","journal-title":"Nature Chemistry"},{"key":"e_1_2_8_11_1","doi-asserted-by":"crossref","first-page":"1729","DOI":"10.1126\/science.1172046","article-title":"Germline P Granules are Liquid Droplets That Localize by Controlled Dissolution\/Condensation","volume":"324","author":"Brangwynne C. P.","year":"2009","journal-title":"Science"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","first-page":"9191","DOI":"10.1074\/jbc.M117.783050","article-title":"Identification of a Multienzyme Complex for Glucose Metabolism in Living Cells","volume":"292","author":"Kohnhorst C. L.","year":"2017","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"2696","DOI":"10.1038\/s41598-018-20348-7","article-title":"A Mathematical Model for Enzyme Clustering in Glucose Metabolism","volume":"8","author":"Jeon M.","year":"2018","journal-title":"Scientific Reports"},{"key":"e_1_2_8_14_1","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1021\/acsbiomedchemau.3c00037","article-title":"Size\u2010Specific Modulation of a Multienzyme Glucosome Assembly During the Cell Cycle","volume":"3","author":"Jeon M.","year":"2023","journal-title":"ACS Bio & Medicinal Chemistry Au"},{"key":"e_1_2_8_15_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2022.101675","article-title":"Subcellular Regulation of Glucose Metabolism Through Multienzyme Glucosome Assemblies by EGF\u2013ERK1\/2 Signaling Pathways","volume":"298","author":"Jeon M.","year":"2022","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_8_16_1","doi-asserted-by":"crossref","first-page":"218a","DOI":"10.1016\/j.bpj.2023.11.1383","article-title":"Hyperosmotic Stress Alters Enzyme Stoichiometry of Glucosomes","volume":"123","author":"Wu H.","year":"2024","journal-title":"Biophysical Journal"},{"key":"e_1_2_8_17_1","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-024-69534-w","article-title":"Mechanistic Insights Into Condensate Formation of Human Liver\u2010Type Phosphofructokinase by Stochastic Modeling Approaches","volume":"14","author":"Kang H.\u2010W.","year":"2024","journal-title":"Scientific Reports"},{"key":"e_1_2_8_18_1","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/j.bpj.2023.02.002","article-title":"Biophysics of Biomolecular Condensates","volume":"122","author":"Joshi A.","year":"2023","journal-title":"Biophysical Journal"},{"key":"e_1_2_8_19_1","doi-asserted-by":"crossref","first-page":"7156","DOI":"10.1021\/acs.biomac.4c00751","article-title":"Exploring LCST\u2010 and UCST\u2010Like Behavior of Branched Molecules Bearing Repeat Units of Elastin\u2010Like Peptides as Side Components","volume":"25","author":"Tanaka N.","year":"2024","journal-title":"Biomacromolecules"},{"key":"e_1_2_8_20_1","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1039\/D5SC07607B","article-title":"Liquid\u2013liquid Phase Separation of Peptides: A Molecular Foundation for next\u2010generation Biomaterials","volume":"17","author":"Lu L.","year":"2026","journal-title":"Chemical Science"},{"key":"e_1_2_8_21_1","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1038\/s41589-024-01806-y","article-title":"The Molecular Mechanism of Temperature\u2010Dependent Phase Separation of Heat Shock Factor 1","volume":"21","author":"Ren Q.","year":"2025","journal-title":"Nature Chemical Biology"},{"key":"e_1_2_8_22_1","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2102772118","article-title":"Local Thermodynamics Govern Formation and Dissolution of Caenorhabditis elegans P Granule Condensates","volume":"118","author":"Fritsch A. W.","year":"2021","journal-title":"Pnas"},{"key":"e_1_2_8_23_1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/s44319-025-00626-y","article-title":"Fluorescent Protein and Peptide Tags Alter Condensate Formation and Dynamics In Vivo and In Vitro","volume":"27","author":"D\u00f6rner K.","year":"2026","journal-title":"Embo Reports"},{"key":"e_1_2_8_24_1","doi-asserted-by":"crossref","first-page":"2305","DOI":"10.1083\/jcb.201701084","article-title":"The Glycolytic Enzyme Phosphofructokinase\u20101 Assembles Into Filaments","volume":"216","author":"Webb B. A.","year":"2017","journal-title":"Journal of Cell Biology"},{"key":"e_1_2_8_25_1","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.15252\/embj.201695957","article-title":"An Aberrant Phase Transition of Stress Granules Triggered by Misfolded Protein and Prevented by Chaperone Function","volume":"36","author":"Mateju D.","year":"2017","journal-title":"Embo Journal"},{"key":"e_1_2_8_26_1","doi-asserted-by":"crossref","first-page":"423","DOI":"10.3389\/fncel.2015.00423","article-title":"Alterations in Stress Granule Dynamics Driven by TDP\u201043 and FUS: A Link to Pathological Inclusions in ALS?","volume":"9","author":"Aulas A.","year":"2015","journal-title":"Frontiers Cellular Neuroscience"},{"key":"e_1_2_8_27_1","doi-asserted-by":"crossref","first-page":"4160","DOI":"10.1093\/hmg\/ddq335","article-title":"Mutant FUS Proteins That Cause Amyotrophic Lateral Sclerosis Incorporate Into Stress Granules","volume":"19","author":"Bosco D. A.","year":"2010","journal-title":"Human Molecular Genetics"},{"key":"e_1_2_8_28_1","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2424135122","article-title":"Material Properties of Biomolecular Condensates Emerge From Nanoscale Dynamics","volume":"122","author":"Galvanetto N.","year":"2025","journal-title":"PNAS"},{"key":"e_1_2_8_29_1","first-page":"143","volume-title":"Methods Enzymol","author":"Alshareedah I.","year":"2021"},{"key":"e_1_2_8_30_1","doi-asserted-by":"crossref","first-page":"3030","DOI":"10.1038\/s41467-022-30521-2","article-title":"Dynamic Arrest and Aging of Biomolecular Condensates are Modulated by Low\u2010Complexity Domains, RNA and Biochemical Activity","volume":"13","author":"Linsenmeier M.","year":"2022","journal-title":"Nature Communications"},{"key":"e_1_2_8_31_1","doi-asserted-by":"crossref","first-page":"3327","DOI":"10.1016\/j.celrep.2018.05.041","article-title":"Different Material States of Pub1 Condensates Define Distinct Modes of Stress Adaptation and Recovery","volume":"23","author":"Kroschwald S.","year":"2018","journal-title":"Cell Reports"},{"key":"e_1_2_8_32_1","doi-asserted-by":"crossref","first-page":"9571","DOI":"10.1021\/jacs.2c13653","article-title":"CAG\u2010Repeat RNA Hairpin Folding and Recruitment to Nuclear Speckles With a Pivotal Role of ATP as a Cosolute","volume":"145","author":"Hautke A.","year":"2023","journal-title":"Journal of the American Chemical Society"},{"key":"e_1_2_8_33_1","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1083\/jcb.151.7.1561","article-title":"In Vivo Analysis of Cajal Body Movement, Separation, and Joining in Live Human Cells","volume":"151","author":"Platani M.","year":"2000","journal-title":"Journal of Cell Biology"},{"key":"e_1_2_8_34_1","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.molcel.2016.02.025","article-title":"Mammalian Heat Shock Response and Mechanisms Underlying Its Genome\u2010wide Transcriptional Regulation","volume":"62","author":"Mahat D. B.","year":"2016","journal-title":"Molecular Cell"},{"key":"e_1_2_8_35_1","doi-asserted-by":"crossref","first-page":"E8194","DOI":"10.1073\/pnas.1706197114","article-title":"Structural and Hydrodynamic Properties of an Intrinsically Disordered Region of a Germ Cell\u2010Specific Protein on Phase Separation","volume":"114","author":"Brady J. P.","year":"2017","journal-title":"Pnas"},{"key":"e_1_2_8_36_1","doi-asserted-by":"crossref","first-page":"2117","DOI":"10.1101\/gad.5.11.2117","article-title":"Attenuation of the Heat Shock Response in HeLa Cells Is Mediated by the Release of Bound Heat Shock Transcription Factor and Is Modulated by Changes in Growth and in Heat Shock Temperatures","volume":"5","author":"Abravaya K.","year":"1991","journal-title":"Genes & Development"},{"key":"e_1_2_8_37_1","doi-asserted-by":"crossref","first-page":"6913","DOI":"10.1039\/C4CC09745A","article-title":"Infrared Laser Triggered Release of Bioactive Compounds From Single Hard Shell Microcapsules","volume":"51","author":"V\u00f6pel T.","year":"2015","journal-title":"Chemical Communications"},{"key":"e_1_2_8_38_1","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1364\/AO.12.000555","article-title":"Optical Constants of Water in the 200\u2010nm to 200\u2010Micrometer Wavelength Region","volume":"12","author":"Hale G. M.","year":"1973","journal-title":"Applied Optics"},{"issue":"36","key":"e_1_2_8_39_1","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1068\/htjr144","article-title":"Characterization of the Thermophysical Properties of Molten Plymers and Liquids Using the Flash Technique","volume":"35","author":"Blumm J.","year":"2003","journal-title":"High Temperatures\u2010High Pressure"},{"key":"e_1_2_8_40_1","doi-asserted-by":"crossref","DOI":"10.1111\/cpr.70122","article-title":"Liquid\u2013Liquid Phase Separation in Major Hallmarks of Cancer","volume":"59","author":"Xie C.","year":"2026","journal-title":"Cell Proliferation"},{"key":"e_1_2_8_41_1","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adg3913","article-title":"Reversible Phase Separation of ESCRT Protein ALIX Through Tyrosine Phosphorylation","volume":"9","author":"Elias R. D.","year":"2023","journal-title":"Science Advances"},{"key":"e_1_2_8_42_1","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1126\/science.abg7071","article-title":"Regulation of Biomolecular Condensates by Interfacial Protein Clusters","volume":"373","author":"Folkmann A. W.","year":"2021","journal-title":"Science"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnano.2025.1556384"},{"key":"e_1_2_8_44_1","doi-asserted-by":"crossref","first-page":"2218","DOI":"10.1039\/C7MB00432J","article-title":"Imperfect Crowding Adaptation of Mammalian Cells Towards Osmotic Stress and Its Modulation by Osmolytes","volume":"13","author":"Gnutt D.","year":"2017","journal-title":"Molecular Biosystems"},{"key":"e_1_2_8_45_1","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1016\/j.cell.2015.07.047","article-title":"A Liquid\u2010to\u2010Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation","volume":"162","author":"Patel A.","year":"2015","journal-title":"Cell"},{"key":"e_1_2_8_46_1","doi-asserted-by":"crossref","first-page":"1910","DOI":"10.1016\/j.jmb.2020.03.004","article-title":"Liquid\u2013Liquid Phase Separation and Its Mechanistic Role in Pathological Protein Aggregation","volume":"432","author":"Babinchak W. M.","year":"2020","journal-title":"Journal of Molecular Biology"},{"key":"e_1_2_8_47_1","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1038\/nmeth.1435","article-title":"Protein Folding Stability and Dynamics Imaged in a Living Cell","volume":"7","author":"Ebbinghaus S.","year":"2010","journal-title":"Nature Methods"},{"key":"e_1_2_8_48_1","doi-asserted-by":"crossref","first-page":"7189","DOI":"10.1073\/pnas.1504822112","article-title":"The Disordered P Granule Protein LAF\u20101 Drives Phase Separation Into Droplets With Tunable Viscosity and Dynamics","volume":"112","author":"Elbaum\u2010Garfinkle S.","year":"2015","journal-title":"PNAS"},{"key":"e_1_2_8_49_1","doi-asserted-by":"crossref","first-page":"9010","DOI":"10.1021\/acs.chemrev.2c00659","article-title":"When Phased Without Water: Biophysics of Cellular Desiccation, From Biomolecules to Condensates","volume":"123","author":"Romero\u2010Perez P. S.","year":"2023","journal-title":"Chemical Reviews"},{"key":"e_1_2_8_50_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2024.136248","article-title":"Macromolecular Crowding Effects on Protein Dynamics","volume":"281","author":"Das N.","year":"2024","journal-title":"International Journal of Biological Macromolecules"},{"key":"e_1_2_8_51_1","doi-asserted-by":"crossref","first-page":"6199","DOI":"10.1038\/s41467-023-41864-9","article-title":"Intermolecular Interactions Underlie Protein\/Peptide Phase Separation Irrespective of Sequence and Structure at Crowded Milieu","volume":"14","author":"Poudyal M.","year":"2023","journal-title":"Nature Communications"},{"key":"e_1_2_8_52_1","doi-asserted-by":"crossref","first-page":"1800","DOI":"10.1093\/bioinformatics\/bts241","article-title":"easyFRAP: An Interactive, Easy\u2010to\u2010Use Tool for Qualitative and Quantitative Analysis of FRAP Data","volume":"28","author":"Rapsomaniki M. A.","year":"2012","journal-title":"Bioinformatics"},{"key":"e_1_2_8_53_1","doi-asserted-by":"crossref","first-page":"7331","DOI":"10.1021\/acs.chemrev.6b00815","article-title":"Single Particle Tracking: From Theory to Biophysical Applications","volume":"117","author":"Shen H.","year":"2017","journal-title":"Chemical Reviews"},{"key":"e_1_2_8_54_1","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1038\/s43586-024-00341-3","article-title":"A Guide to Single\u2010Particle Tracking","volume":"4","author":"Simon F.","year":"2024","journal-title":"Nature Reviews Methods Primers"},{"key":"e_1_2_8_55_1","doi-asserted-by":"crossref","first-page":"10738","DOI":"10.1039\/C6CP08167C","article-title":"Conformational Dynamics and Self\u2010Association of Intrinsically Disordered Huntingtin Exon 1 in Cells","volume":"19","author":"B\u00fcning S.","year":"2017","journal-title":"Physical Chemistry Chemical Physics"},{"key":"e_1_2_8_56_1","doi-asserted-by":"crossref","first-page":"1940","DOI":"10.1002\/2211-5463.13912","article-title":"Heat Application in Live Cell Imaging","volume":"14","author":"Sistemich L.","year":"2024","journal-title":"FEBS Open Bio"},{"key":"e_1_2_8_57_1","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/j.ijthermalsci.2005.11.009","article-title":"Study of the Temperature Field in Microchannels of a PDMS Chip With Embedded Local Heater Using Temperature\u2010Dependent Fluorescent Dye","volume":"45","author":"Fu R.","year":"2006","journal-title":"International Journal of Thermal Sciences"}],"container-title":["Chemistry \u2013 A European Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/chem.71500","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/chem.71500","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/chem.71500","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T10:42:39Z","timestamp":1785753759000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/chem.71500"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,3]]},"references-count":56,"alternative-id":["10.1002\/chem.71500"],"URL":"https:\/\/doi.org\/10.1002\/chem.71500","archive":["Portico"],"relation":{},"ISSN":["0947-6539","1521-3765"],"issn-type":[{"value":"0947-6539","type":"print"},{"value":"1521-3765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,3]]},"assertion":[{"value":"2026-04-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71500"}}