{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T05:52:38Z","timestamp":1789451558446,"version":"build-2803163510"},"reference-count":5,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,3,15]],"date-time":"2004-03-15T00:00:00Z","timestamp":1079308800000},"content-version":"vor","delay-in-days":74,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chirality"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Most HPLC enantioseparations of amine analytes are performed using normal\u2010phase systems containing mobile phases of heptane with ethanol (or 2\u2010propanol) and an amine additive. Since salt\u2010forms of amine analytes are usually insoluble in normal\u2010phase eluents, free\u2010base forms are synthesized for preparative chromatography. It would be highly desirable to directly chromatograph salt forms of amine analytes using mobile phases of carbon dioxide (CO<jats:sub>2<\/jats:sub>) and methanol (MeOH). Such separations would be readily suitable for preparative chromatography, since most amine salts are highly soluble in MeOH. In this article, advantages are shown for the use of supercritical fluid chromatography (SFC) instrumentation with tandem UV and polarimetric detection for confirming enantioseparation as well as for determining optimum preparative column injections. Examples are shown for racemic mixtures of propranolol HCl (I), thioridazine HCl (II), tramadol HCl (III), and flurbiprofen (IV), all of which resolved on Chiralpak AD\u2010H chiral stationary phase using mobile\u2010phase systems of CO<jats:sub>2<\/jats:sub> and MeOH without the use of basic or acidic additives. <jats:italic>Chirality 16:263\u2013266, 2004.<\/jats:italic> \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/chir.20025","type":"journal-article","created":{"date-parts":[[2004,3,17]],"date-time":"2004-03-17T00:01:09Z","timestamp":1079481669000},"page":"263-266","source":"Crossref","is-referenced-by-count":17,"title":["Enantioseparation of hydrochloride salts using carbon dioxide\u2010based mobile phases with on\u2010line polarimetric detection"],"prefix":"10.1002","volume":"16","author":[{"given":"Fiona","family":"Geiser","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rekha","family":"Shah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,3,15]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)01117-6"},{"key":"e_1_2_1_3_2","first-page":"97","article-title":"Enantiomeric quantitation without separation of tramadol via laser\u2010based polarimetry","volume":"1","author":"Edkins TJ","year":"1996","journal-title":"Enantiomer"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)01491-1"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00318-2"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/chir.530060310"}],"container-title":["Chirality"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fchir.20025","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/chir.20025","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T02:03:48Z","timestamp":1696644228000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/chir.20025"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":5,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1002\/chir.20025"],"URL":"https:\/\/doi.org\/10.1002\/chir.20025","archive":["Portico"],"relation":{},"ISSN":["0899-0042","1520-636X"],"issn-type":[{"value":"0899-0042","type":"print"},{"value":"1520-636X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1]]}}}