{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T12:52:57Z","timestamp":1761310377534},"reference-count":12,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,11,8]],"date-time":"2006-11-08T00:00:00Z","timestamp":1162944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chirality"],"published-print":{"date-parts":[[2007,5,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Immobilized lipase from <jats:italic>Candida antarctica<\/jats:italic> (Novozym\u00ae435) was employed in the kinetic resolution of racemic flurbiprofen by enantioselective esterification with methanol. It was found that the lipase has the R\u2010stereopreference and the reaction matches Bi Bi Ping Pong mechanism with dead\u2010end inhibition of methanol. Furthermore, the R\u2010stereopreference was analyzed in details from the aspects of enzymatic kinetic mechanism and reaction activation energy of both enantiomers. The R\u2010enantiomer shows lower activation energy and higher maximum reaction rate than the S\u2010enantiomer, which implies the R\u2010stereopreference of the lipase and makes the kinetic resolution of flurbiprofen via enzymatic reaction feasible. Chirality 2006. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/chir.20347","type":"journal-article","created":{"date-parts":[[2006,11,8]],"date-time":"2006-11-08T14:16:57Z","timestamp":1162995417000},"page":"245-249","source":"Crossref","is-referenced-by-count":11,"title":["R\u2010stereopreference analysis of lipase Novozym\u00ae435 in kinetic resolution of flurbiprofen"],"prefix":"10.1002","volume":"19","author":[{"given":"Hua Yun","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi Bun","family":"Ching","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,11,8]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-199600525-00006"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.197204601"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/chir.530050703"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(99)00154-4"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1252\/jcej.32.338"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1025047410699"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0032-9592(02)00100-0"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1042\/BA20040163"},{"key":"e_1_2_1_10_2","volume-title":"Biochemical engineering fundamentals","author":"Bailey JE","year":"1986"},{"key":"e_1_2_1_11_2","volume-title":"Biochemical Engineering","author":"Blanch HW","year":"1996"},{"key":"e_1_2_1_12_2","volume-title":"Perry's chemical engineering's handbook","author":"Perry RH","year":"1984"},{"key":"e_1_2_1_13_2","volume-title":"Enzyme kinetics","author":"Segal IH","year":"1975"}],"container-title":["Chirality"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fchir.20347","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/chir.20347","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T00:00:49Z","timestamp":1700006449000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/chir.20347"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11,8]]},"references-count":12,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,5,5]]}},"alternative-id":["10.1002\/chir.20347"],"URL":"https:\/\/doi.org\/10.1002\/chir.20347","archive":["Portico"],"relation":{},"ISSN":["0899-0042","1520-636X"],"issn-type":[{"value":"0899-0042","type":"print"},{"value":"1520-636X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,11,8]]}}}