{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T06:34:51Z","timestamp":1700030091409},"reference-count":20,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2007,4,24]],"date-time":"2007-04-24T00:00:00Z","timestamp":1177372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chirality"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A coupled achiral\u2013chiral high performance liquid chromatographic method was developed and fully validated for the determination of bevantolol enantiomers, (\u2212)\u2010(<jats:italic>S<\/jats:italic>)\u2010bevantolol and (+)\u2010(<jats:italic>R<\/jats:italic>)\u2010bevantolol, in human plasma. Plasma samples were prepared by solid phase extraction with Sep\u2010Pak Plus C18 cartridges followed by HPLC. Bevantolol enantiomers and (+)\u2010(<jats:italic>R<\/jats:italic>)\u2010Propranolol as internal standard (IS) were preseparated from interfering components in plasma on a Phenomenex silica column and bevantolol enantiomers and IS were resolved and determined on a Chiralcel OJ\u2010H chiral stationary phase. The two columns were connected by a switching valve equipped with silica precolumn. The Precolumn was used to concentrate bevantolol in the eluent from the achiral column before back flushing onto chiral phase. A detailed validation of the method was performed accordingly to FDA guidelines. For each enantiomer the assay was linear between 20 and 1600 ng\/ml. The quantification limits of both bevantolol enantiomers were 20 ng\/ml. The intraday variation was between 1.07 and 12.64% in relation to the measured concentration and the interday variation was 0.91 and 11.79%. The method has been applied to the determination of (\u2212)\u2010(<jats:italic>S<\/jats:italic>)\u2010 and (+)\u2010(<jats:italic>R<\/jats:italic>)\u2010bevantolol in plasma from healthy volunteers dosed with racemic bevantolol hydrochloride. hydrochloride. Chirality, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/chir.20410","type":"journal-article","created":{"date-parts":[[2007,4,24]],"date-time":"2007-04-24T19:18:06Z","timestamp":1177442286000},"page":"528-535","source":"Crossref","is-referenced-by-count":5,"title":["Determination of bevantolol enantiomers in human plasma by coupled achiral\u2013chiral high performance\u2010liquid chromatography"],"prefix":"10.1002","volume":"19","author":[{"given":"Joung Weon","family":"Oh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tran Quoc","family":"Trung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwan Seog","family":"Sin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong Seong","family":"Kang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyeong Ho","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,4,24]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.1986.150"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-198810010-00001"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/chir.530070803"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1968.tb07949.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.1983.242"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112161"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.1983.240"},{"key":"e_1_2_1_9_2","volume-title":"Drug Stereochemistry: analytical methods and pharmacology","author":"Wainer IW","year":"1993"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(84)80228-5"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)81372-9"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00541549"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00608218"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1130080106"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015858219018"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(97)00338-1"},{"key":"e_1_2_1_17_2","first-page":"53","article-title":"On\u2010line column\u2010switching high performance liquid chromatography analysis of cardiovascular drugs in serum with automated sample clean\u2010up and zone cutting technique to perform chiral separation","volume":"701","author":"Mangani F","year":"1997","journal-title":"J Chromatogr B"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(99)00279-4"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02975242"},{"key":"e_1_2_1_20_2","unstructured":"FDA. Guidance for industry. Bioanalytical method validation; May 2001. Available athttp:\/\/www.fda.gov\/cder\/guidance\/index.htm."},{"key":"e_1_2_1_21_2","unstructured":"FDA. Guidance for industry. Bioavailability and bioequivalence studies for orally administered drug products: General considerations; March 2003. 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