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Pheniramine, oxybutynin, cetirizine, and brinzolamide were resolved using n\u2010hexane\u20102\u2010PrOH\u2010DEA (85:15:0.1, v\/v), n\u2010hexane\u20102\u2010PrOH\u2010DEA (80:20:0.1, v\/v), n\u2010hexane\u20102\u2010PrOH\u2010DEA (70:30:0.2, v\/v), and n\u2010hexane\u20102\u2010propanol (90:10, v\/v) as mobile phases. The separation was carried out at 25\u2009\u00b1\u20091\u2009\u00baC temperature with detection at 225\u2009nm for cetirizine and oxybutynin and 220\u2009nm for pheniramine and brinzolamide. The flow rates of the mobile phases were 0.5 mLmin<jats:sup>\u20101<\/jats:sup>. The retention factors of pheniramine, oxybutynin, cetirizine and brinzolamide were 3.25 and 4.34, 4.76 and 5.64, 6.10 and 6.60, and 1.64 and 2.01, respectively. The separation factors of these drugs were 1.33, 1.18, 1.09 and 1.20 while their resolutions factors were 1.09, 1.45, 1.63 and 1.25, and 1.15, respectively. The absolute configurations of the eluted enantiomers of the reported drugs were determined by simulation studies. It was observed that the order of enantiomers elution of the reported drugs was S\u2010pheniramine\u2009&gt;\u2009R\u2010pheniramine; R\u2010oxybutynin\u2009&gt;\u2009S\u2010oxybutynin; S\u2010cetirizine\u2009&gt;\u2009R\u2010cetirizine; and S\u2010brinzolamide\u2009&gt;\u2009R\u2010brinzolamide. The mechanism of separation was also determined at the supramolecular level by considering interactions and modeling results. The reported SPE\u2010chiral high\u2010performance liquid chromatography<jats:bold>(<\/jats:bold>HPLC) methods are suitable for the enantiomeric analyses of these drugs in any biological sample. In addition, simulation studies may be used to determine the absolute configuration of the first and second eluted enantiomers.<jats:italic>Chirality 26:136\u2013143, 2014<\/jats:italic>. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/chir.22276","type":"journal-article","created":{"date-parts":[[2014,1,27]],"date-time":"2014-01-27T05:13:08Z","timestamp":1390799588000},"page":"136-143","source":"Crossref","is-referenced-by-count":34,"title":["Enantiomeric Separation and Simulation Studies of Pheniramine, Oxybutynin, Cetirizine, and Brinzolamide Chiral Drugs on Amylose\u2010Based Columns"],"prefix":"10.1002","volume":"26","author":[{"given":"Imran","family":"Ali","sequence":"first","affiliation":[{"name":"Department of Chemistry Jamia Millia Islamia, (Central University) New Delhi India"},{"name":"Department of Chemistry, College of Science King Saud University Riyadh Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeid A.","family":"Al\u2010Othman","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science King Saud University Riyadh Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulrahman","family":"Al\u2010Warthan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science King Saud University Riyadh Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Syed Dilshad","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Chemistry Jamia Millia Islamia, (Central University) New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javed A.","family":"Farooqi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, School of Sciences Indira Gandhi National Open University New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,1,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bms262"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1201\/9780203911112"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.21992"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.20397"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/157016307783220576"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.22135"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2008.11.043"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(86)90276-2"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.530040513"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1134"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200500153"},{"key":"e_1_2_9_13_1","first-page":"449","volume-title":"Encyclopedia of chemical processing","author":"Bingyun L","year":"2006"},{"key":"e_1_2_9_14_1","unstructured":"Ding\u2010QiangL XiuLi Yan\u2010LiangL Jia\u2010quanC ShibaT.Resolution of chiral drugs research. 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