{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:05:06Z","timestamp":1787497506354,"version":"build-2736575974"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,3,3]],"date-time":"2014-03-03T00:00:00Z","timestamp":1393804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chirality"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A stereoselective high\u2010performance liquid chromatographic (HPLC) method was developed and validated to determine S\u2010(\u2212)\u2010 and R\u2010(+)\u2010propranolol in rat serum. Enantiomeric resolution was achieved on cellulose tris(3,5\u2010dimethylphenylcarbamate) immobilized onto spherical porous silica chiral stationary phase (CSP) known as Chiralpak IB. A simple analytical method was validated using a mobile phase consisted of n\u2010hexane\u2010ethanol\u2010triethylamine (95:5:0.4%, v\/v\/v) at a flow rate of 0.6\u2009mL\u2009min\n                    <jats:sup>\u20101<\/jats:sup>\n                    and fluorescence detection set at excitation\/emission wavelengths 290\/375\u2009nm. The calibration curves were linear over the range of 10\u2013400\u2009ng\u2009mL\n                    <jats:sup>\u20101<\/jats:sup>\n                    (\n                    <jats:italic>R<\/jats:italic>\n                    \u2009=\u20090.999) for each enantiomer with a detection limit of 3\u2009ng\u2009mL\n                    <jats:sup>\u20101<\/jats:sup>\n                    . The proposed method was validated in compliance with ICH guidelines in terms of linearity, accuracy, precision, limits of detection and quantitation, and other aspects of analytical validation. Actual quantification could be made for propranolol isomers in serum obtained from rats that had been intraperitoneally (i.p.) administered a single dose of the drug. The proposed method established in this study is simple and sensitive enough to be adopted in the fields of clinical and forensic toxicology. Molecular modeling studies including energy minimization and docking studies were first performed to illustrate the mechanism by which the active enantiomer binds to the \u03b2\u2010adrenergic receptor and second to find a suitable interpretation of how both enantiomers are interacting with cellulose tris(3,5\u2010dimethylphenylcarbamate) CSP during the process of resolution. The latter interaction was demonstrated by calculating the binding affinities and interaction distances between propranolol enantiomers and chiral selector.\n                    <jats:italic>Chirality 26:194\u2013199, 2014<\/jats:italic>\n                    . \u00a9 2014 Wiley Periodicals, Inc.\n                  <\/jats:p>","DOI":"10.1002\/chir.22296","type":"journal-article","created":{"date-parts":[[2014,3,3]],"date-time":"2014-03-03T06:46:16Z","timestamp":1393829176000},"page":"194-199","source":"Crossref","is-referenced-by-count":13,"title":["HPLC\u2010Fluorescence Method for the Enantioselective Analysis of Propranolol in Rat Serum Using Immobilized Polysaccharide\u2010Based Chiral Stationary Phase"],"prefix":"10.1002","volume":"26","author":[{"given":"Amer M.","family":"Alanazi","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry College of Pharmacy King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed M.","family":"Hefnawy","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry College of Pharmacy King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulrahman A.","family":"AL\u2010Majed","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry College of Pharmacy King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aymen K.","family":"AL\u2010 Suwailem","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry College of Pharmacy King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed G.","family":"Kassem","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry College of Pharmacy King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gamal A.","family":"Mostafa","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry College of Pharmacy King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabry M.","family":"Attia","sequence":"additional","affiliation":[{"name":"Department of Pharmacology College of Pharmacy King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed M.","family":"Khedr","sequence":"additional","affiliation":[{"name":"Welsh School of Pharmacy Cardiff University UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,3,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/S0022-3565(25)32352-9","article-title":"Stereospecific assay for (\u2212)\u2010 and (+)\u2010 propranolol in human and dog plasma","volume":"215","author":"Silber B","year":"1980","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2008.02.037"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.12.026"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2005.10.017"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.05.092"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.12.090"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1549"},{"key":"e_1_2_7_9_1","volume-title":"3D QSAR in drug design","author":"Kubinyi H","year":"1997"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms13010710"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201000630"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200900738"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.22154"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2012.09.001"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.22084"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2011.12.029"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21334"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1093-3263(99)99999-0"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06325"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.20106"},{"key":"e_1_2_7_21_1","volume-title":"Statistics and chemometrics for analytical chemistry","author":"Miller JN","year":"2005"},{"key":"e_1_2_7_22_1","unstructured":"ICH guideline Q2(R1).Validation of analytical procedures: text and methodology. 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