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A novel and simple liquid chromatographic\u2010electrospray ionization\u2010mass spectrometry (LC\u2010ESI\u2010MS) method was developed for the simultaneous determination of<jats:italic>R<\/jats:italic>\u2010 and<jats:italic>S<\/jats:italic>\u20108PN in rat serum and urine. Carbamazepine was used as the internal standard (IS). Enantiomeric resolution of 8PN was achieved on a Chiralpak<jats:sup>\u00ae<\/jats:sup>AD\u2010RH column with an isocratic mobile phase consisting of 2\u2010propanol and 10\u2009mM ammonium formate (pH\u20098.5) (40:60, v\/v) and a flow rate of 0.7\u2009mL\/min. Detection was achieved using negative selective ion monitoring (SIM) of 8PN at m\/z 339.15 for both enantiomers and positive SIM m\/z at 237.15 for the IS. The calibration curves for urine were linear over a range of 0.01\u201375\u2009\u00b5g\/mL and 0.05\u201375\u2009\u00b5g\/mL for serum with a limit of quantification of 0.05\u2009\u00b5g\/mL in serum and 0.01\u2009\u00b5g\/mL in urine. The method was successfully validated showing that it was sensitive, reproducible, and accurate for enantiospecific quantification of 8PN in biological matrices. The assay was successfully applied to a preliminary study of 8PN enantiomers in rat.<jats:italic>Chirality 26:419\u2013426, 2014<\/jats:italic>. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/chir.22343","type":"journal-article","created":{"date-parts":[[2014,6,14]],"date-time":"2014-06-14T00:35:11Z","timestamp":1402706111000},"page":"419-426","source":"Crossref","is-referenced-by-count":10,"title":["Enantiospecific Analysis of 8\u2010Prenylnaringenin in Biological Fluids by Liquid\u2010Chromatography\u2010Electrospray Ionization Mass Spectrometry: Application to Preclinical Pharmacokinetic Investigations"],"prefix":"10.1002","volume":"26","author":[{"given":"Stephanie E.","family":"Martinez","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy University of Manitoba Winnipeg Manitoba Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ted M.","family":"Lakowski","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy University 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