{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T16:33:36Z","timestamp":1769013216861,"version":"3.49.0"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"State Project for Essential Drug Research and Development","award":["2011ZX09102-001-31"],"award-info":[{"award-number":["2011ZX09102-001-31"]}]},{"name":"Faculty Development fund Project of Guangdong Pharmaceutical University","award":["52104109"],"award-info":[{"award-number":["52104109"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chirality"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A novel method for chiral separation of flurbiprofen enantiomers was developed using aqueous two\u2010phase extraction (ATPE) coupled with biphasic recognition chiral extraction (BRCE). An aqueous two\u2010phase system (ATPS) was used as an extracting solvent which was composed of ethanol (35.0% w\/w) and ammonium sulfate (18.0% w\/w). The chiral selectors in ATPS for BRCE consideration were L\u2010dioctyl tartrate and L\u2010tryptophan, which were screened from amino acids, \u03b2\u2010cyclodextrin derivatives, and L\u2010tartrate esters. Factors such as the amounts of L\u2010dioctyl tartrate and L\u2010tryptophan, pH, flurbiprofen concentration, and the operation temperature were investigated in terms of chiral separation of flurbiprofen enantiomers. The optimum conditions were as follows: L\u2010dioctyl tartrate, 80\u2009mg; L\u2010tryptophan, 40\u2009mg; pH, 4.0; flurbiprofen concentration, 0.10\u2009mmol\/L; and temperature, 25\u2009\u00b0C. The maximum separation factor \u03b1 for flurbiprofen enantiomers could reach 2.34. The mechanism of chiral separation of flurbiprofen enantiomers is discussed and studied. The results showed that synergistic extraction has been established by L\u2010dioctyl tartrate and L\u2010tryptophan, which enantioselectively recognized R\u2010 and S\u2010enantiomers in top and bottom phases, respectively. Compared to conventional liquid\u2013liquid extraction, ATPE coupled with BRCE possessed higher separation efficiency and enantioselectivity without the use of any other organic solvents. The proposed method is a potential and powerful alternative to conventional extraction for separation of various enantiomers. <jats:italic>Chirality 27:650\u2013657, 2015<\/jats:italic>. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/chir.22481","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T20:52:52Z","timestamp":1436907172000},"page":"650-657","source":"Crossref","is-referenced-by-count":23,"title":["Enantioseparation of Racemic Flurbiprofen by Aqueous Two\u2010Phase Extraction With Binary Chiral Selectors of L\u2010dioctyl Tartrate and L\u2010tryptophan"],"prefix":"10.1002","volume":"27","author":[{"given":"Zhi","family":"Chen","sequence":"first","affiliation":[{"name":"College of Pharmacy Guangdong Pharmaceutical University  Guangzhou P.R. 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