{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T02:15:19Z","timestamp":1788574519095,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,12,28]],"date-time":"2016-12-28T00:00:00Z","timestamp":1482883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chirality"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple and efficient high\u2010performance thin\u2010layer chromatographic method was developed for chiral separation of<jats:italic>rac<\/jats:italic>\u2010bupropion (BUP) and its active metabolite<jats:italic>rac<\/jats:italic>\u2010hydroxybupropion (HBUP). Design of experiment (DoE)\u2010based optimization was adopted instead of a conventional trial\u2010and\u2010error approach. The Box\u2013Behnken design surface response model was used and the operating variables were optimized based on 17 trials design. The optimized method involved impregnation of chiral reagent, L(+)\u2010tartaric acid, in the stationary phase with simultaneous addition in the mobile phase, which consisted of acetonitrile : methanol : dichloromethane : 0.50% L\u2010tartaric acid (6.75:1.0:1.0:0.25,<jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>). Under the optimized conditions, the resolution factor between the enantiomers of BUP and HBUP was 6.30 and 9.26, respectively. The limit of detection and limit of quantitation for (R)\u2010BUP, (S)\u2010BUP, (R,R)\u2010HBUP, and (S,S)\u2010HBUP were 9.23 and 30.78\u00a0ng spot<jats:sup>\u22121<\/jats:sup>, 10.32 and 34.40\u00a0ng spot<jats:sup>\u22121<\/jats:sup>, 12.19 and 40.65\u00a0ng spot<jats:sup>\u22121<\/jats:sup>, and 14.26 and 47.53\u00a0ng spot<jats:sup>\u22121<\/jats:sup>, respectively. The interaction of L\u2010tartaric acid with analytes and their retention behavior was thermodynamically investigated using van't Hoff's plots. The developed method was validated as per the International Conference on Harmonization guidelines. Finally, the method was successfully applied to resolve and quantify the enantiomeric content from marketed tablets as well as spiked plasma samples.<\/jats:p>","DOI":"10.1002\/chir.22673","type":"journal-article","created":{"date-parts":[[2016,12,28]],"date-time":"2016-12-28T07:17:58Z","timestamp":1482909478000},"page":"80-88","source":"Crossref","is-referenced-by-count":10,"title":["Design of experiment assisted concurrent enantioseparation of bupropion and hydroxybupropion by high\u2010performance thin\u2010layer chromatography"],"prefix":"10.1002","volume":"29","author":[{"given":"Nejal M.","family":"Bhatt","sequence":"first","affiliation":[{"name":"Chemistry Department, School of Sciences Gujarat University Ahmedabad Gujarat India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijay D.","family":"Chavada","sequence":"additional","affiliation":[{"name":"Chemistry Department, School of Sciences Gujarat University Ahmedabad Gujarat India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mallika","family":"Sanyal","sequence":"additional","affiliation":[{"name":"Chemistry Department St. Xavier's Science College Ahmedabad Gujarat India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1284-1558","authenticated-orcid":false,"given":"Pranav S.","family":"Shrivastav","sequence":"additional","affiliation":[{"name":"Chemistry Department, School of Sciences Gujarat University Ahmedabad Gujarat India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,12,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.59566\/IJBS.2006.2085","article-title":"Chiral drugs: an overview","volume":"2","author":"Nguyen L","year":"2006","journal-title":"Int J Biomed Sci"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.530010103"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/09595230100100642"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.07.007"},{"key":"e_1_2_6_6_1","first-page":"370","article-title":"Resolution of enantiomers of bupropion and its metabolites by liquid chromatography","volume":"30","author":"Batra S","year":"2015","journal-title":"Biomed Chromatogr"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600730820"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.06.009"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-009-1361-z"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2010.11.063"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.01.024"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/34.5.280"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2012.05.008"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1660"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2016.02.032"},{"key":"e_1_2_6_16_1","first-page":"1015","article-title":"Stereoselective method to quantify bupropion and its three major metabolites, hydroxybupropion, erythro\u2010dihydro bupropion, and threo\u2010dihydrobupropion using HPLC\u2013MS\/ MS","author":"Masters AR","year":"2016","journal-title":"J Chromatogr B"},{"key":"e_1_2_6_17_1","unstructured":"CroarkinC TobiasP.NIST\/SEMATECH e\u2010Handbook of Statistical Methods.United States of America:National Institute of Standards and Technology. 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