{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T13:43:44Z","timestamp":1777470224646,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,7,5]],"date-time":"2017-07-05T00:00:00Z","timestamp":1499212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chirality"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A direct fluorometric high\u2010performance liquid chromatography (HPLC) method was developed and validated for the analysis of ibuprofen enantiomers in mouse plasma (100\u00a0\u03bcl) and tissues (brain, liver, kidneys) using liquid\u2013liquid extraction and 4\u2010tertbutylphenoxyacetic acid as an internal standard. Separation of enantiomers was accomplished in a Chiracel OJ\u2010H chiral column based on cellulose tris(4\u2010methylbenzoate) coated on 5\u00a0\u03bcm silica\u2010gel, 250 x 4.6\u00a0mm at 22\u00a0\u00b0C with a mobile phase composed of n\u2010hexane, 2\u2010propanol, and trifluoroacetic acid that were delivered in gradient elution at a flow rate of 1\u00a0ml min<jats:sup>\u22121<\/jats:sup>. A fluorometric detector was set at: \u03bb<jats:sub>excit<\/jats:sub>. = 220\u00a0nm and \u03bb<jats:sub>emis.<\/jats:sub> = 290\u00a0nm. Method validation included the evaluation of the selectivity, linearity, lower limit of quantification (LLOQ), within\u2010run and between\u2010run precision and accuracy. The LLOQ for the two enantiomers was 0.125 \u03bcg ml<jats:sup>\u22121<\/jats:sup> in plasma, 0.09\u00a0\u03bcg g<jats:sup>\u22121<\/jats:sup> in brain, and 0.25\u00a0\u03bcg g<jats:sup>\u22121<\/jats:sup> in for liver and kidney homogenates. The calibration curves showed good linearity in the ranges of each enantiomers: from 0.125 to 35\u00a0\u03bcg ml<jats:sup>\u22121<\/jats:sup> for plasma, 0.09\u20131.44\u00a0\u03bcg g<jats:sup>\u22121<\/jats:sup> for brain, and 0.25\u201320\u00a0\u03bcg g<jats:sup>\u22121<\/jats:sup> for liver and kidney homogenates. The method was successfully applied to a pharmacokinetic study of ibuprofen enantiomers in mice treated i.v. with 10\u00a0mg kg<jats:sup>\u22121<\/jats:sup> of racemate.<\/jats:p>","DOI":"10.1002\/chir.22715","type":"journal-article","created":{"date-parts":[[2017,7,5]],"date-time":"2017-07-05T12:02:48Z","timestamp":1499256168000},"page":"500-511","source":"Crossref","is-referenced-by-count":11,"title":["Enantioselective analysis of ibuprofen enantiomers in mice plasma and tissues by high\u2010performance liquid chromatography with fluorescence detection: Application to a pharmacokinetic study"],"prefix":"10.1002","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9833-5885","authenticated-orcid":false,"given":"Katarzyna","family":"Przejczowska\u2010Pomierny","sequence":"first","affiliation":[{"name":"Department of Pharmacokinetics and Physical Pharmacy Jagiellonian University Medical College  Cracow Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"W\u0142odyka","sequence":"additional","affiliation":[{"name":"Department of Pharmacokinetics and Physical Pharmacy Jagiellonian University Medical College  Cracow Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Cios","sequence":"additional","affiliation":[{"name":"Department of Pharmacokinetics and Physical Pharmacy Jagiellonian University Medical College  Cracow Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"El\u017cbieta","family":"Wyska","sequence":"additional","affiliation":[{"name":"Department of Pharmacokinetics and Physical Pharmacy Jagiellonian University Medical College  Cracow Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,5]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/1099-0801(200006)14:4<219::AID-BMC969>3.0.CO;2-Z"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.1985.tb02694.x"},{"key":"e_1_2_5_4_1","first-page":"237","article-title":"Enantioselective pharmacodynamics and pharmacokinetics of chiral non\u2010steroidal antiinflammatory drugs","volume":"54","author":"Evans AM","year":"1992","journal-title":"Eur J Clin Pharmacol"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03342662"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018969506143"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-199834020-00002"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1213\/ANE.0b013e3181f7b679"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-009-0016-x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2008.01.095"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.111.180224"},{"key":"e_1_2_5_12_1","first-page":"88","article-title":"Pharmacokinetics of ibuprofen enantiomers in rats after intravenous and oral administration of ibuprofen arginate","volume":"47","author":"Wang Y","year":"2012","journal-title":"Acta Pharm Sin"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1081\/DMR-200047999"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1211\/jpp.59.11.0007"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(91)90164-U"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.1995.tb05783.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-636X(1997)9:4<354::AID-CHIR7>3.0.CO;2-D"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-636X(1997)9:3<297::AID-CHIR16>3.0.CO;2-I"},{"key":"e_1_2_5_19_1","first-page":"79","article-title":"HPLC methodology for determination of ibuprofen enantiomers to be used in pharmacokinetics","volume":"43","author":"G\u0142\u00f3wka FK","year":"1998","journal-title":"Chem Anal"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00428-X"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2003.07.010"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1248\/yakushi.125.733"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.01.010"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/47.9.777"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1365"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02490926"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2003.08.031"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.4236\/ajac.2010.12007"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2014.06.024"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201301184"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(93)E0425-P"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2004.11.014"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0731-7085(94)E0012-P"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(97)00212-4"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1081\/JLC-200047202"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-636X(1996)8:1<136::AID-CHIR20>3.0.CO;2-N"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015866704848"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03191018"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02336690"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.530020303"}],"container-title":["Chirality"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fchir.22715","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/chir.22715","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T02:52:55Z","timestamp":1696647175000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/chir.22715"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,5]]},"references-count":39,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1002\/chir.22715"],"URL":"https:\/\/doi.org\/10.1002\/chir.22715","archive":["Portico"],"relation":{},"ISSN":["0899-0042","1520-636X"],"issn-type":[{"value":"0899-0042","type":"print"},{"value":"1520-636X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,5]]}}}