{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T14:46:44Z","timestamp":1788014804768,"version":"build-2784847793"},"reference-count":43,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,30]],"date-time":"2019-10-30T00:00:00Z","timestamp":1572393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81660594, 81360487"],"award-info":[{"award-number":["81660594, 81360487"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chirality"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A novel chiral derivatization reagent, the N\u2010[1\u2010oxo\u20105\u2010(triphenylphosphonium)pentyl]\u2010 (R)\u20101,3\u2010thiazolidinyl\u20104\u2010N\u2010hydroxysuccinimide ester bromide salt (OTPTHE), was developed for the separation and selective detection of chiral DL\u2010amino acids by RP\u2010HPLC analysis. The OTPTHE reacted with DL\u2010amino acids at 60\u00b0C maintained for 30 minutes in the presence of 100 mM borate buffer (pH 9.5). The separability of the diastereomeric derivatives was evaluated in terms of the resolution value (Rs) using 13 kinds of DL\u2010amino acids, which were completely separated by reversed\u2010phase chromatography using C18 column at 254 nm. The Rs of the DL\u2010amino acids varied from 1.62 to 2.51. As for the application of the DL\u2010amino acids, the determination of DL\u2010Ser in the human plasma of healthy volunteers was performed based on our developed method. It was shown that linear calibrations were available with high coefficients of correlation (r<jats:sup>2<\/jats:sup> &gt; 0.9997). The limit of detection (S\/N = 3) of the DL\u2010Ser enantiomers was 5.0 pmol; the relative standard deviations of the intraday and interday variations were below 4.56%; the accuracy ranged between 95.40%\u2010110.06% and 95.45%\u2010109.80%, respectively; the mean recoveries (%) of the DL\u2010Ser spiked in the human plasma were 99.49%\u2010103.74%. The amounts of DL\u2010Ser in the human plasma of healthy volunteers were determined.<\/jats:p>","DOI":"10.1002\/chir.23133","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T05:56:20Z","timestamp":1572501380000},"page":"1043-1052","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Evaluation of chiral separation efficiency of a novel OTPTHE derivatization reagent: Applications to liquid\u2010chromatographic determination of DL\u2010serine in human plasma"],"prefix":"10.1002","volume":"31","author":[{"given":"Yu","family":"Han","sequence":"first","affiliation":[{"name":"Key Laboratory for Natural Resource of Changbai Mountain and Functional Molecules, Ministry of Education College of Pharmacy, Yanbian University  Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei\u2010Na","family":"Jin","sequence":"additional","affiliation":[{"name":"Key Laboratory 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