{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T03:39:21Z","timestamp":1788406761836,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,3,23]],"date-time":"2022-03-23T00:00:00Z","timestamp":1647993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007620","name":"Department of Education of Liaoning Province","doi-asserted-by":"publisher","award":["LJKZ0935"],"award-info":[{"award-number":["LJKZ0935"]}],"id":[{"id":"10.13039\/501100007620","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chirality"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Isoconazole with an asymmetrical carbon is a broad\u2010spectrum antimicrobial imidazole, but there is still lack of relevant report about the potential enantioselectivity in biological samples. The object of this research was to develop and validate a sensitive and effective high performance liquid chromatography\u2010electrospray ionization coupled with tandem mass spectrometry (HPLC\u2010ESI\u2010MS\/MS) method for stereoselective separation and determination of isoconazole enantiomers in Sprague\u2010Dawley (SD) rat plasma and tissues. The greater enantioseparation of isoconazole enantiomers was obtained on a Chiralpak IC column with a mobile phase consisted of acetonitrile\u201010 mM aqueous ammonium acetate (90:10, <jats:italic>v\/v<\/jats:italic>) under the reversed\u2010phase mode. Subsequently, the studied compounds and internal standard (IS) were detected on a multiple reaction monitoring (MRM) mode with positive electrospray ionization source. The experimental and theoretical Electronic Circular Dichroism (ECD) spectra were employed to confirm the absolute configuration of isoconazole enantiomers. Eventually, after full method validation, the newly developed method was successfully applied to the study of enantioselectivity in plasma and tissues in SD rats. Results illustrated that the enantioselective differences in plasma were observed for the evidence that the concentrations of <jats:italic>S<\/jats:italic>\u2010(\u2212)\u2010isoconazole were always higher than <jats:italic>R<\/jats:italic>\u2010(+)\u2010isomer. In terms of tissue distribution, liver, kidney, lung, spleen, and small intestine were the mainly distributed tissues and then followed by heart and muscle. This is the first study to reveal the stereoselective behavior of isoconazole enantiomers in vivo, which also provides reliable and valuable reference for further elucidating the enantioselective metabolisms of isoconazole enantiomers.<\/jats:p>","DOI":"10.1002\/chir.23442","type":"journal-article","created":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T04:04:54Z","timestamp":1648094694000},"page":"901-912","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development of a novel HPLC\u2010ESI\u2010MS\/MS method to analyze the stereoselective pharmacokinetics and tissue distribution of isoconazole enantiomers in rats"],"prefix":"10.1002","volume":"34","author":[{"given":"Yuanyuan","family":"Zhu","sequence":"first","affiliation":[{"name":"School of Pharmacy Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Animal Products and Fishery products Liaoning Institute for Agro\u2010Product Veterinary Drugs and Feed Control, Liaoning Inspection, Examination and Certification Center  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoxian","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Animal Products and Fishery products Liaoning Institute for Agro\u2010Product Veterinary Drugs and Feed Control, Liaoning Inspection, Examination and Certification Center  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine Tsinghua University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5329-9611","authenticated-orcid":false,"given":"Jia","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S155610"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2165\/00128071\u2010200405060\u201000002"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12054"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/abd1806\u20104841.20132018"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2147\/CE.S49629"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2020.461299"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.1992.tb00894.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100507.1994.tb00283.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12055"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0NJ00095G"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201800180"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.07.084"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021\u20109673(94)80443\u20105"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo102459w"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2021.122738"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337\u2010009\u20101106\u2010z"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.22416"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2018.04.027"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0NJ03657A"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2014.06.040"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.07.059"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.7b05446"},{"key":"e_1_2_7_24_1","unstructured":"US Food and Drug Administration.Guidance for industry: bioanalytical method validation.https:\/\/www.fda.gov\/regulatory\u2010information\/search\u2010fda\u2010guidance\u2010documents\/bioanalyticalmethod\u2010validation\u2010guidance\u2010industry. 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