{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T03:45:32Z","timestamp":1793677532938,"version":"4.5.1"},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T00:00:00Z","timestamp":1669939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chirality"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lenalidomide (LND) is an analogue of thalidomide that is second generation immunomodulatory drugs (IMiDs). LND contains asymmetric carbon atom and exist R and S enantiomer. <jats:italic>S<\/jats:italic> (\u2212) form of enantiomer are considered to be more potent and biologically active in tumor cell. It is available in racemic form for clinical use. The study aims to develop and validate enantiomer separation of LND in human plasma. The chromatographic enantiomeric separation was achieved on a Daicel\u2010CSP, Chiralpack IA 4.6\u2009\u00d7\u2009250\u2009mm_5\u2009\u03bcm. The mobile phase was constituted in combination of methanol:glacial acetic acid at a concentration of 499.50\u2009ml: 50\u2009\u03bcl. UV wavelength detection was 220\u2009nm. The RSD% for all validation parameters was found to be within the acceptable limit. The chiral chromatographic (chiral stationary phase\u2013high\u2010performance liquid chromatography [CSP\u2010HPLC]) method developed and validated for the quantitative estimation of LND enantiomers <jats:italic>S<\/jats:italic> (\u2212) and <jats:italic>R<\/jats:italic> (+) in human plasma sample is accurate, precise, robust, stable and selective.<\/jats:p>","DOI":"10.1002\/chir.23520","type":"journal-article","created":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T14:49:05Z","timestamp":1669992545000},"page":"83-91","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Development and validation of a novel chiral chromatographic method for separation of lenalidomide enantiomers in human plasma"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7689-1482","authenticated-orcid":false,"given":"Gyan","family":"Vardhan","sequence":"first","affiliation":[{"name":"Department of Pharmacology All India Institute of Medical Sciences  Rishikesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vikas","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Pharmacology All India Institute of Medical Sciences  Rishikesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puran Lal","family":"Sahu","sequence":"additional","affiliation":[{"name":"National Dope Testing Laboratory  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anuj","family":"Prakash","sequence":"additional","affiliation":[{"name":"Analytical Division, Indian Pharmacopeia Commission  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uttam Kumar","family":"Nath","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology &amp; Hematology All India Institute of Medical Sciences  Rishikesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shailendra","family":"Handu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology All India Institute of Medical Sciences  Rishikesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puneet","family":"Dhamija","sequence":"additional","affiliation":[{"name":"Department of Pharmacology All India Institute of Medical Sciences  Rishikesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737140.6.8.1165"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2012.04.005"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/1538\u20107445.AM2013\u20103357"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27010111"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201701211"},{"key":"e_1_2_7_7_1","unstructured":"ICH Official web site.ICH[Internet]. 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