{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:38:06Z","timestamp":1786977486432,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T00:00:00Z","timestamp":1690502400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008413","name":"University of Dayton","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008413","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chirality"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In this report, we demonstrate that CD spectroscopy can be used as a tool to detect changes to DNA upon irradiation with UV light. We follow the spectroscopic response of DNA samples irradiated at selected exposure times with both CD and UV\u2013Vis spectroscopy. We analyzed four different nucleic acids to evaluate the effect of the sequence on photodegradation. Only one polymer, calf thymus DNA, was a natural nucleic acid and contained all four nucleobases. The other three were synthetic polymers and contained only one type of base pair: poly (deoxyadenylic\u2010deoxythymidylic) acid [poly (dA\u2010dT)\n                    <jats:sub>2<\/jats:sub>\n                    ] and poly (deoxyadenylic acid) \u00b7 poly (deoxythymidylic acid) [poly (dA) \u00b7 poly (dT)], which contained only adenine and thymine; poly (deoxyguanylic\u2010deoxycytidylic) acid [poly (dG\u2010dC)\n                    <jats:sub>2<\/jats:sub>\n                    ], which contained only guanine and cytosine. CD and UV\u2013Vis spectra showed sequence dependent changes. In particular, poly (dA) \u00b7 poly (dT) undergoes changes more rapidly than the other sequences investigated. The CD spectrum of poly (dA) \u00b7 poly (dT) gradually undergoes an inversion, suggesting a change in helicity, before disappearing due to the unfolding of the double strand.\n                  <\/jats:p>","DOI":"10.1002\/chir.23612","type":"journal-article","created":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T08:19:15Z","timestamp":1690532355000},"page":"973-982","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Circular dichroism and UV\u2013Vis detection of UV\u2010induced damage to nucleic acids"],"prefix":"10.1002","volume":"35","author":[{"given":"Reed C.","family":"Dowling","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Dayton  Dayton Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory T.","family":"Carroll","sequence":"additional","affiliation":[{"name":"Aerobiotix, Inc.  Miamisburg Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L.","family":"Kirschman","sequence":"additional","affiliation":[{"name":"Aerobiotix, Inc.  Miamisburg Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark B.","family":"Masthay","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Dayton  Dayton Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6237-8784","authenticated-orcid":false,"given":"Angela","family":"Mammana","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Dayton  Dayton Ohio 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