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A lipase from <jats:italic>Brucella thiophenivorans<\/jats:italic> was prone to exhibit high activity and excellent enantioselectivity toward N\u2010Ac\u2010DL\u2010MetOMe to produce the key chiral intermediate N\u2010acetyl\u2010L\u2010methionine methyl ester (N\u2010Ac\u2010L\u2010MetOMe). The results showed that the enzymatic reaction was carried out in 100\u2009g\/L racemic substrate for 2\u2009h, the conversion reached 51.3%, the enantiomeric excess value N\u2010Ac\u2010L\u2010MetOMe exceeded 99%, and the enantiomeric ratio value &gt;200. Therefore, the lipase from <jats:italic>B.\u00a0thiophenivorans<\/jats:italic> has potential prospects for the resolution of N\u2010Ac\u2010DL\u2010MetOMe to produce the important intermediate N\u2010Ac\u2010L\u2010MetOMe.<\/jats:p>","DOI":"10.1002\/chir.23643","type":"journal-article","created":{"date-parts":[[2024,1,30]],"date-time":"2024-01-30T04:29:59Z","timestamp":1706588999000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Resolution of N\u2010acetyl\u2010DL\u2010methionine methyl ester by the lipase from <i>Brucella thiophenivorans<\/i>"],"prefix":"10.1002","volume":"36","author":[{"given":"Xiaojun","family":"Li","sequence":"first","affiliation":[{"name":"Department of Fundamental Medicine Xinyu University  Xinyu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bioorganic Synthesis of Zhejiang 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