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This study reports the first chiral HPLC method for the simultaneous determination of FIN and its enantiomer, developed and validated using a rigorous analytical quality by design (AQbD) framework. The method employs a CHIRALPAK <jats:sc>AD<\/jats:sc>\u2010H column with an isocratic mobile phase of <jats:italic>n<\/jats:italic>\u2010hexane: ethanol (62:38, v\/v). Optimized conditions provide excellent baseline resolution (R\u209b\u2009=\u20093.5) in under 10\u2009min. The method was validated using accuracy profiles, demonstrating high performance with limits of quantification established at 340\u2009\u03bcg\u2009mL<jats:sup>\u22121<\/jats:sup> for FIN and 0.40\u2009\u03bcg\u2009mL<jats:sup>\u22121<\/jats:sup> for its 4R enantiomer. The AQbD approach successfully defined a robust method operable design region (MODR) to ensure consistent performance. Furthermore, a multimetric sustainability evaluation confirmed the method's favorable eco\u2010profile, achieving a Grade Index (BAGI) score of 77.5 and Red\u2010Green\u2010Blue 12 (RGB12) score of 80.8. This work provides a reliable, robust, and eco\u2010conscious analytical tool essential for the quality control of FIN formulations.<\/jats:p>","DOI":"10.1002\/chir.70052","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T16:09:56Z","timestamp":1756397396000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Robust HPLC Method for the Simultaneous Determination of Finerenone and Its Enantiomer Using AQbD"],"prefix":"10.1002","volume":"37","author":[{"given":"Badriah\u00a0Saad","family":"Al\u2010Farhan","sequence":"first","affiliation":[{"name":"Chemistry Department, Faculty of Science King Khalid University  Abha Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4902-2465","authenticated-orcid":false,"given":"Ghada\u00a0M.\u00a0G.","family":"Eldin","sequence":"additional","affiliation":[{"name":"Egyptian Drug Authority 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