{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T03:55:37Z","timestamp":1776225337755,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chirality"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Eganelisib (EGN, IPI\u2010549), an investigational new drug, is a potent first\u2010in\u2010class PI3K\u03b3 inhibitor with high selectivity compared to other class I PI3K isoforms, with one asymmetric center. Enantiomers have similar physicochemical properties but differ in their biological characteristics, like absorption, distribution, metabolism, and excretion. Hence, a suitable analytical approach is required to investigate the enantioselective behavior in ADME\u2010Tox. In the current study, a sensitive, reliable, and precise chiral LC\u2013MS\/MS method has been developed for quantifying EGN enantiomers in rat plasma, in accordance with USFDA guidelines. Enantiomeric separation was achieved within 15\u2009min on a Chiralpak\u2010IK\u20103 (250\u2009\u00d7\u20094.6\u2009mm, 3\u2009\u03bcm) immobilized polysaccharide column, containing 10\u2009mM ammonium bicarbonate and a premixed mixture of acetonitrile and methanol (80:20\u2009v\/v) as the mobile phase in the ratio of 10:90 (v\/v), with isocratic elution at a flow rate of 0.6\u2009mL\/min. Multiple reaction monitoring (MRM) mode was used to detect EGN and SOF in positive electrospray ionization (ESI) mode, characterized by their transitions at m\/z 529.3\u2192326.3, 161.1, and m\/z 465.1\u2192447.1, 270.2, 252.1. The method demonstrated a linear response in the range of 1\u2013600\u2009ng\/mL for both enantiomers, with a lower limit of quantification of 1\u2009ng\/mL. The validated bioanalytical method was applied to evaluate the enantioselective pharmacokinetics of EGN enantiomers in rat plasma for the first time, utilizing a model\u2010independent approach. The (\n                    <jats:italic>R<\/jats:italic>\n                    )\u2010enantiomer showed relative fold differences in\n                    <jats:italic>C<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    ,\n                    <jats:italic>t<\/jats:italic>\n                    <jats:sub>1\/2<\/jats:sub>\n                    , AUC\n                    <jats:sub>\n                      0\u2010\n                      <jats:italic>t<\/jats:italic>\n                    <\/jats:sub>\n                    , and AUC\n                    <jats:sub>0\u2010\u221e<\/jats:sub>\n                    when compared to the (\n                    <jats:italic>S<\/jats:italic>\n                    )\u2010enantiomer, indicating the enantioselective behavior of EGN enantiomers.\n                  <\/jats:p>","DOI":"10.1002\/chir.70095","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T10:51:43Z","timestamp":1774349503000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enantioselective Chiral Separation and Quantitation of PI3K\u03b3 Inhibitor Eganelisib in Rat Plasma Using UHPLC\u2013MS\/MS: Application to Evaluate Pharmacokinetic Study"],"prefix":"10.1002","volume":"38","author":[{"given":"A.\u00a0R.","family":"Priyadharshni","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Guwahati, Kamrup Assam India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jony\u00a0Susanna","family":"Kandula","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Guwahati, Kamrup Assam 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