{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T09:56:30Z","timestamp":1792835790361,"version":"4.5.0"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007613","name":"Majmaah University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007613","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chirality"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Suzetrigine (SUZ), an analgesic for acute pain, requires strict enantiomeric purity control. This study develops a robust chiral HPLC method for SUZ and its R\u2010enantiomer using analytical quality by design (AQbD). Separation was achieved on a Chiralpak IC\u20103 column with an isocratic n\u2010hexane\u2013ethanol (90:10, v\/v) mobile phase. The optimized method provided baseline resolution (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>s<\/jats:italic>\n                    <\/jats:sub>\n                    \u2009=\u20093.0) in 15\u2009min. Validation via the accuracy profile approach confirmed reliable quantification, with limits of 290\u2009\u03bcg\u2009mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    (SUZ) and 0.32\u2009\u03bcg\u2009mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    (R\u2010enantiomer). The AQbD strategy defined a method operable design region (MODR) ensuring robust routine performance. Furthermore, multimetric sustainability assessments demonstrated a favorable environmental profile, yielding blue applicability grade index (BAGI) and mosaic\u2010WAC scores of 77.5 and 73.2, respectively. This approach enables reliable, eco\u2010friendly enantiomeric purity assessment of SUZ in bulk and pharmaceutical formulations.\n                  <\/jats:p>","DOI":"10.1002\/chir.70118","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T04:44:59Z","timestamp":1782449099000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["AQbD\u2010Guided Chiral HPLC for Enantiomeric Impurity Analysis of Suzetrigine"],"prefix":"10.1002","volume":"38","author":[{"given":"Sultanah M. N.","family":"Alhunayhin","sequence":"first","affiliation":[{"name":"Department of Chemistry, College of Science Al\u2010Zulfi Majmaah University  Al\u2010Majmaah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3057-3016","authenticated-orcid":false,"given":"Ayman M.","family":"Algohary","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science Al\u2010Zulfi Majmaah University  Al\u2010Majmaah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saja A.","family":"Althobaiti","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science and Humanities in Al\u2010Kharj Prince Sattam Bin Abdulaziz University  Al\u2010Kharj Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2591-0946","authenticated-orcid":false,"given":"Abdullah H.","family":"Alluhayb","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science Qassim University  Buraidah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona I.","family":"Shahin","sequence":"additional","affiliation":[{"name":"Biology Department, Faculty of Tymaa Tabuk University  Tabuk Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1095-1072","authenticated-orcid":false,"given":"Ahmed M.","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Egyptian Drug Authority (EDA)  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2985-4208","authenticated-orcid":false,"given":"Tamer H. A.","family":"Hasanin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science Minia University  El\u2010Minia Egypt"},{"name":"Department of Chemistry, College of Science Jouf University  Sakaka Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/15360288.2026.2624502"},{"issue":"6","key":"e_1_2_9_3_1","first-page":"1085","article-title":"Suzetrigine, a Nonopioid NaV1.8 Inhibitor for Treatment of Moderate\u2010to\u2010Severe Acute Pain: Two Phase 3 Randomized Clinical Trials","volume":"142","author":"Bertoch 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