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Chiral analysis can commonly employ hazardous chemicals and large quantities of waste. In this context, this study presents a green chiral high\u2010performance liquid chromatography (HPLC) method for the determination of lumefantrine (LUME) enantiomers. Chromatographic enantioseparation was achieved on a Chiralpak\n                    <jats:sc>AD<\/jats:sc>\n                    column with a mobile phase composed of ethanol at a flow rate of 1\u2009mL\/min. The effects of column temperature on the retention of the enantiomers were investigated, and the analysis temperature was set at 35\u00b0C. The method greenness profile was assessed using the Analytical GREEnness (AGREE) and Analytical Green Star Area (AGSA) metrics by which the method proved to be eco\u2010friendly. The method was validated, showing adequate selectivity, linearity, precision, accuracy, and robustness over the range of 80\u2013120\u2009\u03bcg\/mL per enantiomer. Electronic circular dichroism (ECD) analysis proposed that the first eluted peak was the R\u2010enantiomer and the second eluted peak was the S\u2010enantiomer. Molecular docking studies suggested that LUME may interact mainly through van der Waals and \u03c0\u2010interactions on the Chiralpak\n                    <jats:sc>AD<\/jats:sc>\n                    . A green method was successfully developed for the chiral separation of LUME. The proposed work contributes to more environmentally friendly chiral approaches in pharmaceutical analysis.\n                  <\/jats:p>","DOI":"10.1002\/chir.70122","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T10:53:25Z","timestamp":1784199205000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Green Chiral HPLC Method for Lumefantrine Analysis: Method Development, Greenness Assessment, and Computational Studies"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-2086-3604","authenticated-orcid":false,"given":"Amanda","family":"Mohr","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Controle de Qualidade Farmac\u00eautico (LCQFar), Faculdade de Farm\u00e1cia Universidade Federal do Rio Grande do Sul  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo 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