{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T07:57:32Z","timestamp":1779177452584,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,1,20]],"date-time":"2020-01-20T00:00:00Z","timestamp":1579478400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007655","name":"Aichi Cancer Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007655","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology CR"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Squamous cell papilloma (SCP) is a benign neoplasm of the head and neck. Human papillomavirus (HPV) has been reported to be a tumourigenic factor for SCP. However, not all SCPs are positive for HPV, suggesting that other possible mechanisms are involved in their development. In this study, we examined the mutational status of 51 SCPs using targeted panel sequencing in addition to HPV status using GP5+\/GP6+ PCR. HPV DNA was detected in 6 (12%) SCPs, while <jats:italic>KRAS<\/jats:italic> and <jats:italic>HRAS<\/jats:italic> mutations were detected in 18 (35%) and 17 (33%) SCPs, respectively. Notably, <jats:italic>KRAS<\/jats:italic> mutations, <jats:italic>HRAS<\/jats:italic> mutations and HPV infection were mutually exclusive. The larynx and trachea (4\/7, 57%) were more preferentially infected by HPV than the other sites (2\/44, 5%, <jats:italic>p<\/jats:italic> = 0.0019) and HPV was associated with multifocal development (4\/5, 80%). In contrast, <jats:italic>KRAS<\/jats:italic> and <jats:italic>HRAS<\/jats:italic> mutations in SCPs were evenly distributed across the anatomical sites and found only in single SCPs. In conclusion, this study demonstrated that HPV was not frequently involved in SCPs and that <jats:italic>RAS<\/jats:italic> mutations were more common alterations. In contrast to inverted sinonasal papillomas and oncocytic sinonasal papillomas, SCP may not be a precursor lesion of carcinoma, because these aetiological events in SCP are distinct from squamous cell carcinoma in the same sites.<\/jats:p>","DOI":"10.1002\/cjp2.157","type":"journal-article","created":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T02:36:14Z","timestamp":1579574174000},"page":"154-159","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Frequent <i>KRAS<\/i> and <i>HRAS<\/i> mutations in squamous cell papillomas of the head and neck"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6385-8341","authenticated-orcid":false,"given":"Eiichi","family":"Sasaki","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Diagnostics Aichi Cancer Center Hospital  Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuhiro","family":"Masago","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Diagnostics Aichi Cancer Center Hospital  Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiro","family":"Fujita","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Diagnostics Aichi Cancer Center Hospital  Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuhiro","family":"Hanai","sequence":"additional","affiliation":[{"name":"Department of Head and Neck Surgery Aichi Cancer Center Hospital  Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1788-559X","authenticated-orcid":false,"given":"Yasushi","family":"Yatabe","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Diagnostics Aichi Cancer Center Hospital  Nagoya 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