{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:43:43Z","timestamp":1791229423351,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T00:00:00Z","timestamp":1614297600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004622","name":"KWF Kankerbestrijding","doi-asserted-by":"publisher","award":["10438"],"award-info":[{"award-number":["10438"]}],"id":[{"id":"10.13039\/501100004622","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology CR"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tumor\u2010derived cell\u2010free DNA (cfDNA) is an emerging biomarker for guiding the personalized treatment of patients with metastatic colorectal cancer (CRC). While patients with CRC liver metastases (CRC\u2010LM) have relatively high levels of plasma cfDNA, little is known about patients with CRC peritoneal metastases (CRC\u2010PM). This study evaluated the presence of tumor\u2010derived cfDNA in plasma and peritoneal fluid (i.e. ascites or peritoneal washing) in 20 patients with isolated CRC\u2010PM and in the plasma of 100 patients with isolated CRC\u2010LM. Among tumor tissue <jats:italic>KRAS\/BRAF<\/jats:italic> mutation carriers, tumor\u2010derived cfDNA was detected by droplet digital polymerase chain reaction (ddPCR) in plasma of 93% of CRC\u2010LM and 20% of CRC\u2010PM patients and in peritoneal fluid in all CRC\u2010PM patients. Mutant allele fraction (MAF) and mutant copies per ml (MTc\/ml) were lower in CRC\u2010PM plasma than in CRC\u2010LM plasma (median MAF = 0.28 versus 18.9%, <jats:italic>p<\/jats:italic> &lt;\u20090.0001; median MTc\/ml = 21 versus 1,758, <jats:italic>p<\/jats:italic> &lt;\u20090.0001). Within patients with CRC\u2010PM, higher cfDNA levels were observed in peritoneal fluid than in plasma (median MAF = 16.4 versus 0.28%, <jats:italic>p<\/jats:italic> =\u20090.0019; median MTc\/ml = 305 versus 21, <jats:italic>p<\/jats:italic> =\u20090.0034). These data imply that tumor\u2010derived cfDNA in plasma is a poor biomarker to monitor CRC\u2010PM. Instead, cfDNA detection in peritoneal fluid may offer an alternative to guide CRC\u2010PM treatment decisions.<\/jats:p>","DOI":"10.1002\/cjp2.207","type":"journal-article","created":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T08:39:23Z","timestamp":1614328763000},"page":"203-208","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Detection of tumor\u2010derived cell\u2010free <scp>DNA<\/scp> from colorectal cancer peritoneal metastases in plasma and peritoneal fluid"],"prefix":"10.1002","volume":"7","author":[{"given":"Iris","family":"van't Erve","sequence":"first","affiliation":[{"name":"Department of Pathology The Netherlands Cancer Institute  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koen P","family":"Rovers","sequence":"additional","affiliation":[{"name":"Department of Surgery Catharina Cancer Institute  Eindhoven The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Constantinides","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology, Division of Imaging and Cancer UMC Utrecht  Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Bolhuis","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Amsterdam University Medical Centers  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma CE","family":"Wassenaar","sequence":"additional","affiliation":[{"name":"Department of Surgery St. Antonius Hospital  Nieuwegein The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin J","family":"Lurvink","sequence":"additional","affiliation":[{"name":"Department of Surgery Catharina Cancer Institute  Eindhoven The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9ment J","family":"Huysentruyt","sequence":"additional","affiliation":[{"name":"Department of Pathology Laboratory for Pathology and Medical Microbiology (PAMM)  Eindhoven The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petur","family":"Snaebjornsson","sequence":"additional","affiliation":[{"name":"Department of Pathology The Netherlands Cancer Institute  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Djamila","family":"Boerma","sequence":"additional","affiliation":[{"name":"Department of Surgery St. Antonius Hospital  Nieuwegein The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daan","family":"van den Broek","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine The Netherlands Cancer Institute  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tineke E","family":"Buffart","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Oncology The Netherlands Cancer Institute  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max J","family":"Lahaye","sequence":"additional","affiliation":[{"name":"Department of Radiology The Netherlands Cancer Institute  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arend GJ","family":"Aalbers","sequence":"additional","affiliation":[{"name":"Department of Surgery The Netherlands Cancer Institute  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels FM","family":"Kok","sequence":"additional","affiliation":[{"name":"Department of Surgery The Netherlands Cancer Institute  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerrit 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