{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:17:29Z","timestamp":1792109849415,"version":"4.4.0"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T00:00:00Z","timestamp":1616371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology CR"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gastric cancer is a heterogenous disease with different phenotypes, genotypes, and clinical outcomes, including sensitivity to treatments and prognoses. Recent medical advances have enabled the classification of this heterogenous disease into several groups and the consequent analysis of their clinicopathological characteristics. Gastric cancer associated with Epstein\u2013Barr virus (EBV) and microsatellite\u2010unstable tumors are considered to be the two major subtypes as they are clearly defined by well\u2010established methodologies, such as <jats:italic>in situ<\/jats:italic> hybridization and polymerase chain reaction\u2010based analyses, respectively. However, discrepancies in the histological diagnosis of gastric neoplasms remain problematic, and international harmonization should be performed to improve our understanding of gastric carcinogenesis. We re\u2010evaluated Japanese cases of early gastric cancer according to the current World Health Organization (WHO) criteria and classified them into genomic subtypes based on microsatellite instability (MSI) and EBV positivity to determine the initial genetic events in gastric carcinogenesis. A total of 113 Japanese early gastric cancers (including low\u2010 and high\u2010grade dysplasias) treated with endoscopic resection over 5\u2009years were archived in our hospital. A histological re\u2010evaluation according to the WHO criteria revealed 54 adenocarcinomas, which were divided into 6 EBV\u2010positive (11.1%), 7 MSI\u2010high (MSI\u2010H, 13.0%), and 41 microsatellite stable cases (75.9%). MSI\u2010H adenocarcinoma was confirmed by an immunohistochemistry assay of mismatch repair proteins. Programmed death\u2010ligand 1 immunostaining with two antibodies (E1L3N and SP263) was positive in tumor cells of one MSI\u2010H adenocarcinoma case (1\/7, 14.3%). The proportion of stained cells was higher with clone SP263 than with E1L3N. Histologically, EBV\u2010positive carcinomas were poorly differentiated (83.8%), and MSI\u2010H cancers were frequent in well to moderately differentiated adenocarcinoma (85.7%), indicating that the EBV\u2010positive subtype presented with high\u2010grade morphology even when an early lesion. Our study indicates that the WHO criteria are useful for subdividing Japanese early gastric cancers, and this subdivision may be useful for comparative analysis of precursor lesions and early carcinoma.<\/jats:p>","DOI":"10.1002\/cjp2.209","type":"journal-article","created":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T07:07:20Z","timestamp":1616396840000},"page":"397-409","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Clinicopathological characteristics of <scp>Epstein\u2013Barr<\/scp> virus and microsatellite instability subtypes of early gastric neoplasms classified by the Japanese and the World Health Organization criteria"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9029-5081","authenticated-orcid":false,"given":"Hiroki","family":"Tanabe","sequence":"first","affiliation":[{"name":"Division of Gastroenterology and Hematology\/Oncology, Department of Medicine 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