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Additionally, GCG can morphologically closely resemble other giant cell\u2010rich lesions, including non\u2010ossifying fibroma (NOF), aneurysmal bone cyst (ABC), giant cell tumour of bone (GCTB), and chondroblastoma. The epigenetic basis of these giant cell\u2010rich tumours is unclear and DNA methylation profiling has been shown to be clinically useful for the diagnosis of other tumour types. Therefore, we aimed to assess the DNA methylation profile of central and peripheral sporadic GCG and cherubism to test whether DNA methylation patterns can help to distinguish them. Additionally, we compared the DNA methylation profile of these lesions with those of other giant cell\u2010rich mimics to investigate if the microscopic similarities extend to the epigenetic level. DNA methylation analysis was performed for central (<jats:italic>n<\/jats:italic>\u2009=\u200910) and peripheral (<jats:italic>n<\/jats:italic>\u2009=\u200910) GCG, cherubism (<jats:italic>n<\/jats:italic>\u2009=\u20096), NOF (<jats:italic>n<\/jats:italic>\u2009=\u200910), ABC (<jats:italic>n<\/jats:italic>\u2009=\u200916), GCTB (<jats:italic>n<\/jats:italic>\u2009=\u20099), and chondroblastoma (<jats:italic>n<\/jats:italic>\u2009=\u200910) using the Infinium Human Methylation EPIC Chip. Central and peripheral sporadic GCG and cherubism share a related DNA methylation pattern, with those of peripheral GCG and cherubism appearing slightly distinct, while central GCG shows overlap with both of the former. NOF, ABC, GCTB, and chondroblastoma, on the other hand, have distinct methylation patterns. The global and enhancer\u2010associated CpG DNA methylation values showed a similar distribution pattern among central and peripheral GCG and cherubism, with cherubism showing the lowest and peripheral GCG having the highest median values. By contrast, promoter regions showed a different methylation distribution pattern, with cherubism showing the highest median values. In conclusion, DNA methylation profiling is currently not capable of clearly distinguishing sporadic and cherubism\u2010associated giant cell lesions. Conversely, it could discriminate sporadic GCG of the jaws from their giant cell\u2010rich mimics (NOF, ABC, GCTB, and chondroblastoma).<\/jats:p>","DOI":"10.1002\/cjp2.337","type":"journal-article","created":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T04:54:36Z","timestamp":1691556876000},"page":"464-474","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<scp>DNA<\/scp> methylation profile discriminates sporadic giant cell granulomas of the jaws and cherubism from their giant cell\u2010rich histological mimics"],"prefix":"10.1002","volume":"9","author":[{"given":"Let\u00edcia Martins","family":"Guimar\u00e3es","sequence":"first","affiliation":[{"name":"Department of Pathology, Biological Science Institute Universidade Federal de Minas Gerais (UFMG)  Belo Horizonte Minas Gerais 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