{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T15:07:27Z","timestamp":1785683247034,"version":"3.56.0"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,12,4]],"date-time":"2017-12-04T00:00:00Z","timestamp":1512345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Pathology CR"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Zika virus (ZIKV) infection during pregnancy can cause adverse fetal outcomes and severe irreversible congenital birth defects including microcephaly. Immunohistochemistry (IHC) is a valuable diagnostic tool for detecting ZIKV antigens in tissues from cases of fetal loss in women infected with ZIKV, and for providing insights into disease pathogenesis. As a result, there is increasing demand for commercially available ZIKV antibodies for use in IHC assays. ZIKV antibodies were selected and obtained from commercial sources to include both mouse and rabbit hosts, and a variety of antigenic targets. Pretreatment conditions and antibody concentrations resulting in optimal immunohistochemical staining were determined using ZIKV cell control and polymerase chain reaction (PCR)\u2010confirmed ZIKV case control material (fetal brain tissue). Cross\u2010reactivity of the antibodies against other flaviviruses (dengue virus serogroups 1\u20134, yellow fever virus, Japanese encephalitis virus, West Nile virus) and chikungunya virus was also evaluated. Immunostaining using the commercially available antibodies was compared to a previously validated ZIKV IHC assay used for primary diagnosis. Four antibodies demonstrated optimal staining similar to the previously validated ZIKV IHC assay. Two of the four antibodies cross\u2010reacted with dengue virus, while the other two antibodies showed no cross\u2010reactivity with dengue, other flaviviruses, or chikungunya virus. Differences in the cross\u2010reactivity profiles could not be entirely explained by the antigenic target. Commercially available ZIKV antibodies can be optimized for use in IHC testing to aid in ZIKV diagnostic testing and an evaluation of tissue tropism.<\/jats:p>","DOI":"10.1002\/cjp2.84","type":"journal-article","created":{"date-parts":[[2017,10,5]],"date-time":"2017-10-05T10:00:33Z","timestamp":1507197633000},"page":"19-25","source":"Crossref","is-referenced-by-count":23,"title":["Optimization of commercially available Zika virus antibodies for use in a laboratory\u2010developed immunohistochemical assay"],"prefix":"10.1002","volume":"4","author":[{"given":"Brigid C","family":"Bollweg","sequence":"first","affiliation":[{"name":"Infectious Diseases Pathology Branch Centers for Disease Control and Prevention Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana","family":"Silva\u2010Flannery","sequence":"additional","affiliation":[{"name":"Infectious Diseases Pathology Branch Centers for Disease Control and Prevention Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Spivey","sequence":"additional","affiliation":[{"name":"Infectious Diseases Pathology Branch Centers for Disease Control and Prevention Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8935-9981","authenticated-orcid":false,"given":"Gillian L","family":"Hale","sequence":"additional","affiliation":[{"name":"Infectious Diseases Pathology Branch Centers for Disease Control and Prevention Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60238-X"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0805715"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMsr1604338"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMe1601862"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2016-0397-SA"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2016-0406-SA"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-017-0749-x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1590\/0074-02760160085"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2016.10.024"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)30883-2"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6506e1"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2303.161499"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-016-0543-1"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-016-1417-2"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep00028"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870501700509"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1354\/vp.42-4-405"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870501700407"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature18952"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3527(03)59002-9"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00291-4"}],"container-title":["The Journal of Pathology: Clinical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcjp2.84","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cjp2.84","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T21:14:54Z","timestamp":1760303694000},"score":1,"resource":{"primary":{"URL":"https:\/\/pathsocjournals.onlinelibrary.wiley.com\/doi\/10.1002\/cjp2.84"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,4]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1002\/cjp2.84"],"URL":"https:\/\/doi.org\/10.1002\/cjp2.84","archive":["Portico"],"relation":{},"ISSN":["2056-4538","2056-4538"],"issn-type":[{"value":"2056-4538","type":"print"},{"value":"2056-4538","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,4]]}}}