{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T05:07:05Z","timestamp":1792732025962,"version":"4.5.0"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,12,5]],"date-time":"2006-12-05T00:00:00Z","timestamp":1165276800000},"content-version":"vor","delay-in-days":978,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Cardiology"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Background<\/jats:italic>: Chronic mental and physical stress has been suggested to be a trigger for cardiovascular events. In addition, a reduction in levels of intracellular magnesium has been reported to cause vasoconstriction while enhancing platelet\u2010dependent thrombosis.<\/jats:p><jats:p><jats:italic>Hypothesis<\/jats:italic>: The purpose of this study was to investigate whether chronic stress affects endothelial function and intra\u2010cellular magnesium levels in humans.<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>: Flow\u2010mediated dilation (endothelium\u2010dependent vasodilation) and sublingual nitroglycerin\u2010induced dilation (0.3 mg, endothelium\u2010independent vasodilation) were measured in the brachial artery in 30 healthy male college students, aged 22 \u00b1 1 years, using high\u2010resolution ultrasound both before and immediately after a 4\u2010week final term examination period. Erythrocyte magnesium concentration was measured simultaneously. All students had chronic sleep deprivation for 4 weeks, during which sleep lasted &lt; 80% of that on ordinary days; in addition, the students were under great stress to pass the examination. This condition was considered to be chronic stress.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>: Chronic stress decreased flow\u2010mediated dilation and erythrocyte magnesium concentration (from 7.4 \u00b1 3.0 to 3.7 \u00b1 2.3%, p &lt; 0.05; from 5.7 \u00b1 0.4 to 5.5 \u00b1 0.4 mg\/ml, p &lt; 0.05, respectively). The change in flow\u2010mediated dilation correlated significantly with that of the erythrocyte magnesium concentration (r = 0.43, p &lt; 0.05), but not with nitroglycerin\u2010induced dilation.<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>: Chronic stress was found to attenuate endo\u2010thelial function, which may also be associated with a reduction in the intracellular magnesium level in humans.<\/jats:p>","DOI":"10.1002\/clc.4960270411","type":"journal-article","created":{"date-parts":[[2007,6,27]],"date-time":"2007-06-27T20:54:30Z","timestamp":1182977670000},"page":"223-227","source":"Crossref","is-referenced-by-count":90,"title":["Effect of chronic stress and sleep deprivation on both flow\u2010mediated dilation in the brachial artery and the intracellular magnesium level in humans"],"prefix":"10.1002","volume":"27","author":[{"given":"Bonpei","family":"Takase","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Akima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akimi","family":"Uehata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumitaka","family":"Ohsuzu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Kurita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,12,5]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.99.16.2192"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(84)91106-1"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-8703(84)90302-8"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1253\/jcj.62.341"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7361117"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/clc.4960200315"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1161\/01.CIR.83.2.391","article-title":"Modulation of coronary vasomotor tone in humans. Progressive endothelial dysfunction with different early stages of coronary atherosclerosis","volume":"83","author":"Zeiher AM","year":"1991","journal-title":"Circulation"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(95)00327-4"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9149(98)00702-4"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/1358863X9700200203"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.102.20.2473"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-8703(84)90572-6"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2000.278.3.R628"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1291\/hypres.23.669"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-4975(98)00020-4"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF03029740"},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1055\/s-0038-1650528","article-title":"Magnesium inhibits platelet activity\u2010an in vitro study","volume":"76","author":"Ravn HB","year":"1996","journal-title":"Thromb Haemost"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1992.00400230015003"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)81098-7"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.68.5.1270"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116772"},{"issue":"1","key":"e_1_2_1_23_2","first-page":"R75","article-title":"Chronic stress affects immunologic but not cardiovascular responsiveness to acute psychological stress in humans","volume":"266","author":"Benschop RJ","year":"1994","journal-title":"Am J Physiol"}],"container-title":["Clinical Cardiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fclc.4960270411","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/clc.4960270411","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T02:16:13Z","timestamp":1700273773000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/clc.4960270411"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,4]]}},"alternative-id":["10.1002\/clc.4960270411"],"URL":"https:\/\/doi.org\/10.1002\/clc.4960270411","archive":["Portico"],"relation":{},"ISSN":["0160-9289","1932-8737"],"issn-type":[{"value":"0160-9289","type":"print"},{"value":"1932-8737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,4]]}}}