{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T04:32:56Z","timestamp":1789619576137,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T00:00:00Z","timestamp":1626912000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["EXC2151 (ID 390873048)"],"award-info":[{"award-number":["EXC2151 (ID 390873048)"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB1403 (ID 414786233)"],"award-info":[{"award-number":["SFB1403 (ID 414786233)"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["TRR237 (ID 369799452)"],"award-info":[{"award-number":["TRR237 (ID 369799452)"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational All"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The pathogenesis of contact dermatitis, a common inflammatory skin disease with limited treatment options, is held to be driven by inflammasome activation induced by allergens and irritants. We here aim to identify inflammasome\u2010targeting treatment strategies for irritant contact dermatitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A high content screen with 41,184 small molecules was performed using fluorescent Apoptosis associated speck\u2010like protein containing a CARD (ASC) speck formation as a readout for inflammasome activation. Hit compounds were validated for inhibition of interleukin (IL)\u20101\u03b2 secretion. Of these, the approved thiuramdisulfide derivative disulfiram was selected and tested in a patch test model of irritant contact dermatitis in 25 healthy volunteers. Topical application of disulfiram, mometasone or vehicle was followed by application of sodiumdodecylsulfate (SDS) for 24\u00a0h each. Eczema induction was quantified by mexameter and laser speckle imaging. Corneocyte sampling of lesional skin was performed to assess inflammasome\u2010mediated cytokines IL\u20101\u03b2 and IL\u201018.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Disulfiram induced a dose\u2010dependent inhibition of ASC speck formation and IL\u20101\u03b2 release in cellular assays in vitro. In vivo, treatment with disulfiram, but not with vehicle and less mometasone, inhibited SDS\u2010induced eczema. This was demonstrated by significantly lower erythema and total perfusion values assessed by mexameter and laser speckle imaging for disulfiram compared to vehicle (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001) and\/or mometasone (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001). Also, corneocyte IL\u201018 levels were significantly reduced after application of disulfiram compared to vehicle (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We show that disulfiram is a dose\u2010dependent inhibitor of inflammasome pathway activation in vitro and inhibitor of SDS\u2010induced eczema in vivo. Topical application of disulfiram represents a potential treatment option for irritant contact dermatitis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/clt2.12045","type":"journal-article","created":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T18:35:31Z","timestamp":1626978931000},"source":"Crossref","is-referenced-by-count":19,"title":["Topical inflammasome inhibition with disulfiram prevents irritant contact dermatitis"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3567-0149","authenticated-orcid":false,"given":"Hanna","family":"Bonnekoh","sequence":"first","affiliation":[{"name":"Dermatological Allergology, Allergie\u2010Centrum\u2010Charit\u00e9, Department of Dermatology, Venereology and Allergology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health  Berlin Germany"},{"name":"Autoinflammation Reference Center Charit\u00e9 (ARC2) Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Vera","sequence":"additional","affiliation":[{"name":"Dermatological Allergology, Allergie\u2010Centrum\u2010Charit\u00e9, Department of Dermatology, Venereology and Allergology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health  Berlin Germany"},{"name":"Autoinflammation Reference Center Charit\u00e9 (ARC2) Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Abad\u2010Perez","sequence":"additional","affiliation":[{"name":"Dermatological Allergology, Allergie\u2010Centrum\u2010Charit\u00e9, Department of Dermatology, Venereology and Allergology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health  Berlin Germany"},{"name":"Autoinflammation Reference Center Charit\u00e9 (ARC2) Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke","family":"Radetzki","sequence":"additional","affiliation":[{"name":"Department of Chemical Biology Leibniz\u2010Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Neuenschwander","sequence":"additional","affiliation":[{"name":"Department of Chemical Biology Leibniz\u2010Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgar","family":"Specker","sequence":"additional","affiliation":[{"name":"Department of Chemical Biology Leibniz\u2010Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niklas Amadeus","family":"Mahnke","sequence":"additional","affiliation":[{"name":"Dermatological Allergology, Allergie\u2010Centrum\u2010Charit\u00e9, Department of Dermatology, Venereology and Allergology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health  Berlin Germany"},{"name":"Autoinflammation Reference Center Charit\u00e9 (ARC2) Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Frischbutter","sequence":"additional","affiliation":[{"name":"Dermatological Allergology, Allergie\u2010Centrum\u2010Charit\u00e9, Department of Dermatology, Venereology and Allergology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health  Berlin Germany"},{"name":"Autoinflammation Reference Center Charit\u00e9 (ARC2) Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eicke","family":"Latz","sequence":"additional","affiliation":[{"name":"Institute of Innate Immunity University of Bonn  Bonn Germany"},{"name":"German Center of Neurodegenerative Diseases (DZNE) University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Nazar\u00e9","sequence":"additional","affiliation":[{"name":"Department of Chemical Biology Leibniz\u2010Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens v.","family":"Kries","sequence":"additional","affiliation":[{"name":"Department of Chemical Biology Leibniz\u2010Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus","family":"Maurer","sequence":"additional","affiliation":[{"name":"Dermatological Allergology, Allergie\u2010Centrum\u2010Charit\u00e9, Department of Dermatology, Venereology and Allergology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health  Berlin Germany"},{"name":"Autoinflammation Reference Center Charit\u00e9 (ARC2) Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Scheffel","sequence":"additional","affiliation":[{"name":"Dermatological Allergology, Allergie\u2010Centrum\u2010Charit\u00e9, Department of Dermatology, Venereology and Allergology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health  Berlin Germany"},{"name":"Autoinflammation Reference Center Charit\u00e9 (ARC2) Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karoline","family":"Krause","sequence":"additional","affiliation":[{"name":"Dermatological Allergology, Allergie\u2010Centrum\u2010Charit\u00e9, Department of Dermatology, Venereology and Allergology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health  Berlin Germany"},{"name":"Autoinflammation Reference Center Charit\u00e9 (ARC2) Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2003.05601.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.14167"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ddg.12510_1"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12973"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12038"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2020.03.014"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-021-00271-4"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700819"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-013-9759-z"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.09-RAI-0160"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.10.003"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-523-1_8"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11030-009-9187-z"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3806"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-24029-3"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.117.013755"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genom-083117-021452"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1517\/17460441.2016.1165203"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2020.03.007"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2018.97"},{"issue":"3","key":"e_1_2_8_22_1","doi-asserted-by":"crossref","first-page":"274","DOI":"10.2340\/0001555559274276","article-title":"Treatment of scabies with disulfiram and benzyl benzoate emulsion: a controlled study","volume":"59","author":"Landegren J","year":"1979","journal-title":"Acta Derm Venereol"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3109\/02713683.2011.622853"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.1773"},{"issue":"9","key":"e_1_2_8_25_1","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1111\/j.1610-0387.2009.06787.x","article-title":"Performing patch testing with contact allergens","volume":"6","author":"Schnuch A","year":"2008","journal-title":"J Dtsch Dermatol Ges"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2004-818963"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1159\/000085865"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0909-752X.2006.00168.x"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/ics.12149"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-1064"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1983.tb04394.x"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1982.tb04137.x"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.4103\/0378-6323.25635"},{"key":"e_1_2_8_34_1","volume-title":"The MAK\u2010Collection for Occupational Health and Safety","year":"1997"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1995.tb00471.x"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/1046-199X(95)90118-3"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-020-0669-6"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20082481"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/000155500750042899"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)90837-8"}],"container-title":["Clinical and Translational Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/clt2.12045","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/clt2.12045","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/clt2.12045","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T20:58:20Z","timestamp":1693342700000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/clt2.12045"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7]]},"references-count":39,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["10.1002\/clt2.12045"],"URL":"https:\/\/doi.org\/10.1002\/clt2.12045","archive":["Portico"],"relation":{},"ISSN":["2045-7022","2045-7022"],"issn-type":[{"value":"2045-7022","type":"print"},{"value":"2045-7022","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7]]},"article-number":"e12045"}}