{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:45:30Z","timestamp":1761864330172,"version":"3.41.2"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,8,17]],"date-time":"2021-08-17T00:00:00Z","timestamp":1629158400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational All"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Environmental exposure chambers (EECs) have been used extensively to study allergic rhinoconjunctivitis. Few studies have been published using EECs in conjunctivitis only, and none have used conjunctival allergen challenge as a selection criterion. The present study validated ALYATEC EEC in allergic conjunctivitis to birch pollen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sixteen patients with a positive conjunctival allergen challenge (CAC) were exposed to 60\u00a0ng\/m<jats:sup>3<\/jats:sup> of Bet v 1 in an EEC on two consecutive days for a maximum of 4\u00a0h to validate EEC exposure to birch. Reproducibility was tested\u00a0among seven of the patients. A conjunctival positive scoring during the CAC and the EEC exposure was defined as a Total Ocular Symptom Score (TOSS)\u00a0\u2265\u00a05.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fifty percent of patients had a conjunctival positive scoring during first exposure and 75% during second exposure. The mean time to a conjunctival response was 81.2\u00a0\u00b1\u00a033.9\u00a0min and 101.6\u00a0\u00b1\u00a057 (<jats:italic>P<\/jats:italic>\u00a0&gt;\u00a00.05) during first and second exposure, respectively. No difference in TOSS occurred between the two exposures. The time necessary to obtain a positive response during the CAC was significantly shorter than with the EEC. The estimated quantity of Bet v 1 inducing a positive response was 0.07\u00a0\u00b1\u00a00.03\u00a0ng (exposure 1), 0.07\u00a0\u00b1\u00a00.07\u00a0ng (exposure 2), 980\u00a0\u00b1\u00a0784\u00a0ng (CAC). Conjunctival positive scoring and quantity of Bet v 1 was reproducible in all six EEC exposures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Early conjunctival responses induced by birch allergen exposures in EEC were different than from those identified with direct instillation during CAC. EEC appears to be closer to natural exposure than CAC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/clt2.12053","type":"journal-article","created":{"date-parts":[[2021,8,17]],"date-time":"2021-08-17T08:04:45Z","timestamp":1629187485000},"source":"Crossref","is-referenced-by-count":11,"title":["Birch allergen challenges in allergic conjunctivitis using standard conjunctival allergen challenge and environmental exposure chamber"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3198-1396","authenticated-orcid":false,"given":"Alina","family":"Gherasim","sequence":"first","affiliation":[{"name":"ALYATEC Environmental Exposure Chamber  Strasbourg France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Luc","family":"Fauquert","sequence":"additional","affiliation":[{"name":"University Hospital  Clermont\u2010Ferrand France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Domis","sequence":"additional","affiliation":[{"name":"ALYATEC Environmental Exposure Chamber  Strasbourg France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xavier","family":"Siomboing","sequence":"additional","affiliation":[{"name":"Soladis Clinical Studies  Roubaix France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederic","family":"de Blay","sequence":"additional","affiliation":[{"name":"ALYATEC Environmental Exposure Chamber  Strasbourg France"},{"name":"Chest Diseases Department Strasbourg University Hospital  Strasbourg France"},{"name":"University of Strasbourg  Strasbourg France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,8,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12009"},{"issue":"376","key":"e_1_2_8_3_1","first-page":"459","article-title":"Management of ophthalmic dis\u2010ease in general practice","volume":"43","author":"Sheldrick JH","year":"1993","journal-title":"Br J Gen Pract"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.315.7111.816"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2500\/aap.2014.35.3750"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000355630"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2007.01620.x"},{"key":"e_1_2_8_8_1","first-page":"36","article-title":"Skin prick tests with standardized extracts of inhalant aller\u2010gens in 7,099 adult participants with asthma or rhinitis: cross\u2010sensitizations and relationships to age, sex, month of birth and year of testing","volume":"6","author":"Eriksson NE","year":"1996","journal-title":"J Investig Allergol Clin Immunol"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2006.01293.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.14111"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1990.01070030090035"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12986"},{"key":"e_1_2_8_13_1","first-page":"379","article-title":"Dose\u2010dependent pro\u2010tection by azelastine eye drops against pollen\u2010induced allergic conjunctivitis. 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