{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T19:27:08Z","timestamp":1791487628831,"version":"4.3.4"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T00:00:00Z","timestamp":1655164800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational All"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Mast cells (MC) from systemic mastocytosis (SM) patients release MC mediators that lead to an altered microenvironment with potential consequences on innate immune cells, such as monocytes and dendritic cells (DC). Here we investigated the distribution and functional behaviour of different populations of blood monocytes and DC among distinct diagnostic subtypes of SM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Overall, we studied 115 SM patients \u2010 45 bone marrow mastocytosis (BMM), 61 indolent SM (ISM), 9 aggressive SM (ASM)\u2010 and 32 healthy donors (HD). Spontaneous and <jats:italic>in vitro<\/jats:italic>\u2010stimulated cytokine production by blood monocytes, and their plasma levels, together with the distribution of different subsets of blood monocytes and DCs, were investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>SM patients showed increased plasma levels and spontaneous production by blood monocytes of IL1\u03b2, IL6, IL8, TNF\u03b1 and IL10, associated with an exhausted ability of LPS\u00a0+\u00a0IFN\u03b3\u2010stimulated blood monocytes to produce IL1\u03b2 and TGF\u03b2. SM (particularly ISM) patients also showed decreased counts of total monocytes, at the expense of intermediate monocytes and non\u2010classical monocytes. Interestingly, while ISM and ASM patients had decreased numbers of plasmacytoid DC and myeloid DC (and their major subsets) in blood, an expansion of AXL<jats:sup>+<\/jats:sup> DC was specifically encountered in BMM cases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results demonstrate an altered distribution of blood monocytes and DC subsets in SM associated with constitutive activation of functionally impaired blood monocytes and increased plasma levels of a wide variety of inflammatory cytokines, reflecting broad activation of the innate immune response in mastocytosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/clt2.12167","type":"journal-article","created":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T07:47:18Z","timestamp":1655192838000},"source":"Crossref","is-referenced-by-count":9,"title":["Altered innate immune profile in blood of systemic mastocytosis 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