{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:59:24Z","timestamp":1785423564768,"version":"3.56.0"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T00:00:00Z","timestamp":1654646400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018537","name":"National Science and Technology Major Project","doi-asserted-by":"publisher","award":["2019ZX09201\u2010005"],"award-info":[{"award-number":["2019ZX09201\u2010005"]}],"id":[{"id":"10.13039\/501100018537","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational All"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hypoxia is considered a key factor in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). However, the specific mechanism driving polypogenesis under hypoxic conditions is unclear. This study aimed to explore hypoxia\u2010induced alterations in the transcriptome of human nasal epithelial cells (HNECs) in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>HNECs derived from the tissue of patients with CRSwNP were established as air\u2013liquid interface (ALI) cultures. Confluent cultures were kept submerged or treated with cobalt chloride (CoCl2) to induce hypoxia. Transcriptome analysis was used to identify key mRNAs involved in this process. Real\u2010time PCR (RT\u2013PCR), Western blotting, and immunofluorescence were used to observe the effects of hypoxia on ciliogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Numerous genes, biological processes and pathways were altered under submerged culture conditions or after CoCl2 treatment. Analysis of the results under both hypoxic conditions revealed that the transcriptional program responsible for ciliogenesis was significantly impaired. Downregulation of cilia\u2010related genes and inhibition of ciliated cell differentiation under hypoxia were confirmed by RT\u2013PCR, Western blot and immunofluorescence analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Hypoxia impairs ciliogenesis and ciliary function in HNECs, which might play a role in the pathogenesis of CRSwNP.<\/jats:p><\/jats:sec>","DOI":"10.1002\/clt2.12168","type":"journal-article","created":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T07:39:25Z","timestamp":1654673965000},"source":"Crossref","is-referenced-by-count":10,"title":["Transcriptome sequencing reveals altered ciliogenesis under hypoxia in nasal epithelial cells from chronic rhinosinusitis with nasal polyps"],"prefix":"10.1002","volume":"12","author":[{"given":"Jian","family":"Jiao","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Beijing TongRen Hospital Capital Medical University  Beijing 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