{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T09:21:26Z","timestamp":1771492886409,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,23]],"date-time":"2025-02-23T00:00:00Z","timestamp":1740268800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004329","name":"Javna Agencija za Raziskovalno Dejavnost RS","doi-asserted-by":"publisher","award":["P3\u20100360"],"award-info":[{"award-number":["P3\u20100360"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004329","name":"Javna Agencija za Raziskovalno Dejavnost RS","doi-asserted-by":"publisher","award":["51977"],"award-info":[{"award-number":["51977"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational All"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>IL\u201033 is involved in allergic processes by promoting the release of various mast cell (MC) chemokines, including CCL2. However, it is yet unclear which specific cell type is primarily responsible for producing CCL2 during acute allergic reactions. This study aims to investigate the role of IL\u201033 in promoting CCL2 production in mast cells and assess the effect of MC\u2010derived CCL2 on basophil migration and endothelial permeability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human blood\u2010derived MCs (hMCs) were generated from peripheral blood precursors, passively sensitized with IgE, treated with IL\u201033, and stimulated with anti\u2010IgE. The concentrations of nine cytokines known to influence immune cell chemotaxis (CCL2, CCL5, CCL11, MIP\u20101\u03b1, IL\u20108, IL\u201010, IL\u201013, granulocyte\u2010macrophage colony\u2010stimulating factor (GM\u2010CSF), and vascular endothelial growth factor (VEGF) were assessed in the supernatants of hMCs. Subsequently, we investigated the impact of MC\u2010derived CCL2 on basophil migration in vitro, as well as its effect on endothelial monolayer permeability using human umbilical vein endothelial cells (HUVECs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Stimulation with anti\u2010IgE induced a significant release of CCL2, GM\u2010CSF, IL\u20108 and VEGF from hMCs. Additionally, incubation with IL\u201033 overnight increased the production of several cytokines. Mast cell\u2010derived CCL2 not only enhanced basophil migration in vitro but also increased endothelial monolayer permeability in HUVECs. The effect was reversed by a C\u2013C chemokine receptor type 2 (CCR2) antagonist, indicating the involvement of CCL2 signaling through the CCR2 receptor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>IL\u201033 induces the production of chemotactic cytokines in hMCs. Mast cell\u2010derived CCL2 plays an important role in basophil chemotaxis in vitro and affects endothelial monolayer permeability in the HUVEC model.<\/jats:p><\/jats:sec>","DOI":"10.1002\/clt2.70044","type":"journal-article","created":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T08:32:15Z","timestamp":1740558735000},"source":"Crossref","is-referenced-by-count":4,"title":["CCL2\/C\u2013C chemokine receptor type 2\u2010mediated interactions among mast cells, basophils, and endothelial cells"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5471-8538","authenticated-orcid":false,"given":"Maru\u0161a","family":"Rihar","sequence":"first","affiliation":[{"name":"University Clinic of Respiratory and Allergic Diseases Golnik  Golnik Slovenia"},{"name":"Biotechnical Faculty University of Ljubljana  Ljubljana 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