{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:17:42Z","timestamp":1791908262125,"version":"4.3.4"},"reference-count":47,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T00:00:00Z","timestamp":1754265600000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016036","name":"Health~Holland","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016036","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015084","name":"Lung Foundation Netherlands","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100015084","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational All"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Microbial dysbiosis is an important feature in allergic asthma. Short\u2010chain fatty acids (SCFA) produced by the intestinal microbiome play a role in the gut\u2010lung axis. Little is known about how the gut SCFA levels reflect SCFA levels in other tissues and how these link to the allergic asthma inflammatory status.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Male BALB\/c mice were intranasally exposed to house dust mite (HDM) to induce allergic airway inflammation. Acetate, propionate, and butyrate levels were determined in caecum content, serum and lungs of control and HDM\u2010allergic mice using liquid chromatography\u2010mass spectrometry. Faecal microbiome composition was determined by DNA sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mean acetate:propionate:butyrate ratios were 75:15:10 in caecum content, 98:1.5:0.5 in serum, and 38:61:1 in the lung. SCFA levels did not correlate across compartments and propionate was relatively high in the lungs. The faecal microbiome of allergic mice differed from control, with increased Desulfovibrionaceae abundance. The lung acetate proportion was lower in allergic mice compared to control. In allergic mice, declining lung acetate levels correlated with increased HDM\u2010specific IgE in serum and IL\u201013 release by ex vivo HDM\u2010restimulated lung cells. Ex vivo acetate supplementation did not inhibit HDM\u2010restimulated lung cell IL\u201013 production, while butyrate and propionate did.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Overall, HDM\u2010driven murine allergic airway inflammation induced changes in the faecal microbiome and reduced acetate in serum and lung tissue. Hereby, lung acetate levels correlated negatively with sensitisation and type\u20102 inflammation, but acetate itself did not suppress ex vivo HDM\u2010induced cytokine release. Our findings provide new insights on the systemic effects of HDM\u2010allergic inflammation along the gut\u2010lung axis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/clt2.70082","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T04:29:38Z","timestamp":1754368178000},"source":"Crossref","is-referenced-by-count":5,"title":["Lung Acetate Levels Decline in Correlation With Increased Type 2 Allergic Markers in a House Dust Mite Allergic Mouse Model"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7830-3603","authenticated-orcid":false,"given":"Roos E. 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