{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T17:58:33Z","timestamp":1788026313730,"version":"build-2784847793"},"reference-count":67,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T00:00:00Z","timestamp":1754611200000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008207","name":"AstraZeneca Canada","doi-asserted-by":"publisher","award":["ESR\u201016\u201012430"],"award-info":[{"award-number":["ESR\u201016\u201012430"]}],"id":[{"id":"10.13039\/100008207","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational All"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by tissue eosinophilia and itch. We evaluated the effect of eosinophil depletion with benralizumab on markers of inflammation in the skin of patients with AD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>After a 4\u2010week washout from oral anti\u2010inflammatory medications 20 patients with moderate to severe AD completed a randomized, double\u2010blind, placebo\u2010controlled parallel\u2010group study comparing 3 doses q4wk 30\u00a0mg subcutaneous benralizumab to placebo. Lesional and unaffected skin biopsies were collected before and after 28 and 65\u00a0days of treatment, respectively, for quantification of eosinophils and IL\u20105R\u03b1\u2010bearing cells in papillary dermis. Histological measurements of epidermal thickness, spongiosis, neutrophilic and lymphocytic infiltration, as well as clinical scores EASI, SCORAD, IGA, DLQI, and POEM were conducted throughout the study. Outcomes were compared between placebo and benralizumab treatment groups using a Mann\u2010Whitney <jats:italic>U<\/jats:italic>\u2010test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Benralizumab, compared to placebo, reduced IL\u20105R\u03b1+ cells and MBP+ EG2+ eosinophils in lesions and unaffected skin (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). In skin lesions, benralizumab reduced MBP+ eosinophils and basophils but had no effect on eosinophil progenitor (EoP; CD34+ IL\u20105R\u03b1+) or mast cell numbers. There was no change in other skin histological measurements or IGA scoring of the observational lesion, nor improvement in clinical scores.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Benralizumab treatment significantly inhibited accumulation of MBP+ eosinophils and basophils in lesional skin of patients with moderate to severe AD. However, a lack of improvement in histological and clinical outcomes suggests that other inflammatory pathways are central to the pathobiology of severe atopic dermatitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Trial Registration<\/jats:title><jats:p>(ClincialTrials.gov number, NCT03563066)<\/jats:p><\/jats:sec>","DOI":"10.1002\/clt2.70090","type":"journal-article","created":{"date-parts":[[2025,8,9]],"date-time":"2025-08-09T04:10:19Z","timestamp":1754712619000},"source":"Crossref","is-referenced-by-count":5,"title":["Benralizumab Depletes IL\u20105R\u03b1\u2010Bearing Cells in Skin Lesions of Patients With Atopic Dermatitis"],"prefix":"10.1002","volume":"15","author":[{"given":"Christiane E.","family":"Whetstone","sequence":"first","affiliation":[{"name":"Department of Medicine Division of Respirology McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth 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