{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T22:32:25Z","timestamp":1791930745476,"version":"4.3.4"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp;amp; Translational All"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Subcutaneous immunotherapy (SCIT) has been a cornerstone treatment for allergic rhinitis (AR) for over 50\u00a0years, consistently demonstrating symptom reduction and modulation of immune responses. Despite this, the underlying mechanisms responsible for the efficacy of SCIT remain incompletely understood, especially with regard to local immune responses in lymph nodes and nasal mucosa.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To determine the impact of SCIT treatment on immunoglobulin production in blood and nasal mucosa, as well as B cell class\u2010switching in blood and lymph nodes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Blood, nasal lavage (NAL), and fine\u2010needle aspirates (FNA) of inguinal lymph nodes were collected from 23 patients with birch and\/or timothy pollen\u2010induced AR before and after SCIT updosing. General response to SCIT was evaluated with symptom and medication scores, as well as allergen\u2010mediated activation of basophils. Allergen\u2010specific IgE, IgA, IgG, and IgG4 were measured in blood and NAL via ELISA. B\u2010cell class\u2010switching was assessed in blood and FNAs by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>AR symptoms, medication use, and basophil sensitivity to allergens was reduced after SCIT updosing. Interestingly, the plasma levels of allergen\u2010specific IgA, IgG, and IgG4 increased after SCIT updosing, while the levels of allergen\u2010specific IgE and IgA decreased in NAL at this timepoint. Moreover, these results were accompanied by an increase in conventional (IgD<jats:sup>\u2212<\/jats:sup>CD27<jats:sup>+<\/jats:sup>) and unconventional (IgD<jats:sup>\u2212<\/jats:sup>CD27<jats:sup>\u2212<\/jats:sup>) memory B cells in blood and lymph nodes, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study highlights the differential effects of SCIT on local and systemic immunity and identifies early immunological changes associated with treatment. However, confirmation of long\u2010term tolerance will require extended follow\u2010up, including in\u2010season analyses. The local decrease in allergen\u2010specific IgA in NAL, alongside a systemic increase in allergen\u2010specific IgA and IgG4, underscores the importance of assessing both mucosal and systemic responses when evaluating SCIT efficacy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/clt2.70097","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T11:50:37Z","timestamp":1756813837000},"source":"Crossref","is-referenced-by-count":2,"title":["Subcutaneous Allergen\u2010Specific Immunotherapy for Allergic Rhinitis: Divergent IgA Responses in Nasal Mucosa and Blood With Validation of B Cell Class\u2010Switching in Lymph Nodes and Blood"],"prefix":"10.1002","volume":"15","author":[{"given":"Maryam","family":"Jafari","sequence":"first","affiliation":[{"name":"Division of Ear, Nose, and Throat Diseases Department of Clinical Science Intervention and Technology Karolinska Institutet  Stockholm 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