{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T07:17:15Z","timestamp":1787815035819,"version":"build-2784847793"},"reference-count":56,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp;amp; Translational All"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Shellfish allergy is the most common food allergy in adults and the third most common in children. \u03b3\u03b4 T cells have been identified as playing a critical role in antigen tolerance in allergic diseases in mouse models. In humans, \u03b3\u03b4 T cells may play a regulatory role in peanut immunotherapy, and their role in shrimp allergy remains unclear. We hypothesized \u03b3\u03b4 T cells play a regulatory role in shrimp allergic disease. We performed single cell RNA sequencing (scRNAseq) on peripheral cells from shrimp allergic (SA) and healthy control (HC) subjects after stimulation with shrimp tropomyosin. This revealed a significant expansion of \u03b3\u03b4 T cells with three distinct clusters. One \u03b3\u03b4 T cell cluster predominated in SA, characterized as CD8+ with a cytotoxic expression profile. We found significant upregulation of TGF\u2010\u03b21 and downregulation of IL\u20107R in SA\u2010stimulated \u03b3\u03b4 T cells, and IL\u201010RA expression in stimulated SA total PBMCs. \u03b3\u03b4 T cells may play a role in shrimp allergic disease through lymphocyte\u2010mediated cytotoxin signaling and cytokine\u2010mediated signaling pathways, including TGF\u03b2\u20101, IL7\/TSLP\u2010IL7R, and IL10\u2010IL10R pathways.<\/jats:p>","DOI":"10.1002\/clt2.70189","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T03:46:25Z","timestamp":1785469585000},"source":"Crossref","is-referenced-by-count":0,"title":["Gamma Delta T Cells in Shrimp Allergy Express a Unique Cytotoxic Cytokine Profile"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7236-9857","authenticated-orcid":false,"given":"Brenda Bin","family":"Su","sequence":"first","affiliation":[{"name":"Immunology, Allergy, and Retrovirology Division of the Department of Pediatrics at Baylor College of Medicine William T. Shearer Center for Human Immunobiology Texas Children's Hospital  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2988-4340","authenticated-orcid":false,"given":"Tyler","family":"Jackson","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Huffington Center on Aging Baylor College of Medicine  Houston Texas USA"},{"name":"Program in Cancer Cell Biology Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Warren","family":"Blackmon","sequence":"additional","affiliation":[{"name":"Immunology, Allergy, and Retrovirology Division of the Department of Pediatrics at Baylor College of Medicine William T. Shearer Center for Human Immunobiology Texas Children's Hospital  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harold","family":"Ames","sequence":"additional","affiliation":[{"name":"Immunology, Allergy, and Retrovirology Division of the Department of Pediatrics at Baylor College of Medicine William T. Shearer Center for Human Immunobiology Texas Children's Hospital  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Holt","sequence":"additional","affiliation":[{"name":"Immunology, Allergy, and Retrovirology Division of the Department of Pediatrics at Baylor College of Medicine William T. Shearer Center for Human Immunobiology Texas Children's Hospital  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aikaterini","family":"Anagnostou","sequence":"additional","affiliation":[{"name":"Immunology, Allergy, and Retrovirology Division of the Department of Pediatrics at Baylor College of Medicine William T. Shearer Center for Human Immunobiology Texas Children's Hospital  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vibha","family":"Szafron","sequence":"additional","affiliation":[{"name":"Immunology, Allergy, and Retrovirology Division of the Department of Pediatrics at Baylor College of Medicine William T. Shearer Center for Human Immunobiology Texas Children's Hospital  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9942-6188","authenticated-orcid":false,"given":"Sara","family":"Anvari","sequence":"additional","affiliation":[{"name":"Immunology, Allergy, and Retrovirology Division of the Department of Pediatrics at Baylor College of Medicine William T. Shearer Center for Human Immunobiology Texas Children's Hospital  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjie","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Huffington Center on Aging Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0866-7822","authenticated-orcid":false,"given":"Carla M.","family":"Davis","sequence":"additional","affiliation":[{"name":"Immunology, Allergy, and Retrovirology Division of the Department of Pediatrics at Baylor College of Medicine William T. Shearer Center for Human Immunobiology Texas Children's Hospital  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2018.5630"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2019.12.027"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.iac.2025.04.002"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2019.07.031"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2022.12.827"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3FO04440H"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2015.02.001"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2018.04.008"},{"key":"e_1_2_11_10_1","first-page":"467","article-title":"Identification of Tropomyosin and Arginine Kinase as Major Allergens of Portunus pelagicus (Blue Swimming Crab)","volume":"29","author":"Rosmilah M.","year":"2012","journal-title":"Tropical Biomedicine"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2019.11.022"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27092667"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000065172"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2013.02.043"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.11.017"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.14632"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/gene.2014.45"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.06\u20102434"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2021.108820"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.15250"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165\u20102478(95)02451\u20104"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.28.584"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20103083.2007.01963.x"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0804288"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc\u20102023\u2010007981"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25031381"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201051794\u20101"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1282758"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1110267"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0810059106"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13302"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijd.16364"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.13.7451"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0091\u20106749(03)01865\u20107"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.04.048"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201021246\u20109"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/database\/baz046"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2400114"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.12561"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.16627"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aba8310"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398\u20109995.2010.02479.x"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.10.002"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201546204"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.12.1711"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.12.1711"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.11.6720"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071681"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20090153"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.1.225"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/pai.13407"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI157962"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.add3085"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1293158"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201021702\u20106"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326\u20106066.CIR\u201021\u20100539"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00851"}],"container-title":["Clinical and Translational Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/clt2.70189","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/clt2.70189","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/clt2.70189","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T06:21:50Z","timestamp":1787811710000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/clt2.70189"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,30]]},"references-count":56,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/clt2.70189"],"URL":"https:\/\/doi.org\/10.1002\/clt2.70189","archive":["Portico"],"relation":{},"ISSN":["2045-7022","2045-7022"],"issn-type":[{"value":"2045-7022","type":"print"},{"value":"2045-7022","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,30]]},"article-number":"e70189"}}