{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T16:53:21Z","timestamp":1759683201764},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,4,9]],"date-time":"2002-04-09T00:00:00Z","timestamp":1018310400000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The presence of Myosin Va (an actin\u2010based molecular motor) in the peripheral nervous system was examined and its subcellular distribution within the axons of the sciatic nerve was demonstrated via immunocytochemistry. Myosin Va (M\u2010Va) in the nerve was detected by using SDS\u2010PAGE and Western blot techniques with a polyclonal antibody specifically raised against the M\u2010Va globular tail domain. In addition, purification of M\u2010Va from the rat sciatic nerve prior to immunoblotting yielded a M\u2010Va standard band. Likewise, optical immunocytochemical procedures revealed the presence of M\u2010Va, particularly in the cortical axoplasmic territory, but also in the Schwann cell soma. The above experiments were carried out both on intact as well as on severed sciatic nerves with similar results. The proximal stumps of severed sciatic nerves (from 0 to 72 h after injury) were labelled in vivo with <jats:sup>35<\/jats:sup>S\u2010methionine. SDS\u2010PAGE autoradiography of the immunoabsorbed M\u2010Va from the radiolabelled homogenized nerve tissue showed a significant increment of the radioactive intensity of M\u2010Va heavy chain band through time. Moreover, a significant increment of transcripts coding for M\u2010Va heavy chain was detected through time using RT\u2010PCR after nerve injury and compared to intact nerves. This data suggest that M\u2010Va is up\u2010regulated in a time\u2010dependent manner. The latter suggests a possible involvement of M\u2010Va in nerve regeneration processes. Cell Motil. Cytoskeleton 51:169\u2013176, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cm.10017","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T23:24:15Z","timestamp":1030317855000},"page":"169-176","source":"Crossref","is-referenced-by-count":19,"title":["Myosin Va is locally synthesized following nerve 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